DuoSet® IC (Intracellular) Phospho-specific ELISA Development Systems

The DuoSet IC Phospho-specific ELISA format is a sensitive and convenient phosphorylation assay for use with cell lysates. Extensive validation work is done in-house to ensure specificity. These signal transduction assays are an excellent alternative to Western blot, especially when used in combination with DuoSet IC ELISAs designed to measure the levels of total protein.


  • Optimized capture and detection antibody pairings and recommended concentrations save lengthy development time
  • Generic development protocols provide direction to start an optimization protocol
  • Customize the assay to your specific needs
  • Available in 2, 5, and 15-(96-well) plate pack sizes
  • Excellent alternative to Western blot

Assay Principle

View Full Assay Principle

Kit Contents

Kit contents may vary. Please refer to product-specific inserts for a complete list of contents for individual assays.

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard
  • Streptavidin-HRP