DuoSet® IC (IntraCellular) Assay Development Systems
DuoSet IC ELISA Reagents are sensitive and convenient assays used to measure intracellular protein levels in cell lysates. Extensive validation work is done to ensure specificity, and detailed protocols are provided. R&D Systems offers DuoSet IC assays to measure kinases, apoptosis-related and genotoxic stress proteins, heat shock proteins, and more. These signal transduction assays make an excellent alternative to Western blot, especially when used in combination with R&D Systems DuoSet IC Phospho-specific ELISAs.
Features
- Optimized capture and detection antibody pairings and recommended concentrations save lengthy development time
- Mass-calibrated standards allow for consistent results and eliminate vial-to-vial variation
- Generic development protocols provide direction to start an optimization protocol
- Customize the assay to your specific needs
- Available in 2, 5, and 15-(96-well) plate pack sizes
- Excellent alternative to Western blot
Kit Contents
Kit contents may vary slightly. Please refer to product-specific inserts for a complete list of contents for individual assays.
- Capture Antibody
- Conjugated Detection Antibody
- Calibrated Immunoassay Standard
- Streptavidin-HRP
Related Information
- Apoptosis
- Apoptosis Intracellular Kinases
- Apoptosis Transcription Factors and Regulators
- Caspases and Regulators
- ELISA Development Guide
- Genotoxic Stress Response/Cell Cycle
- Heat Shock Proteins (HSPs)
- Intracellular Kinases
- Intracellular Kinases in the Akt Pathway
- Mini-review: Methods for Detecting Protein Phosphorylation
- Phosphatases and Regulators
- Phosphatases in the Akt Pathway
- Receptor Tyrosine Kinases (RTKs)
- Receptor Tyrosine Kinases (RTKs) in the Akt Pathway
- Stem Cell Transcription Factor Regulators
- Transcription Factors and Regulators
- Transcription Factors in the Akt Pathway
- Transcriptional Co-Activators
- Transcriptional Co-Repressors
- Troubleshooting Guide: ELISA Development