DYKDDDDK Epitope Tag Antibody (C.15200054) - BSA Free

Novus Biologicals | Catalog # NBP2-59146

Novus Biologicals
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Key Product Details

Species Reactivity

Epitope Tag

Applications

Western Blot, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # C.15200054

Format

BSA Free
View all DYKDDDDK Epitope Tag Primary Antibodies »

Product Specifications

Immunogen

The exact immunogen of DYKDDDDK Epitope Tag Antibody (C.15200054) is propietary information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

1.01 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DYKDDDDK Epitope Tag Antibody (C.15200054) - BSA Free

Western Blot: DYKDDDDK Epitope Tag Antibody (C.15200054) [NBP2-59146]

Western Blot: DYKDDDDK Epitope Tag Antibody (C.15200054) [NBP2-59146]

Western Blot: DYKDDDDK Epitope Tag Antibody (C.15200054) [NBP2-59146] - Analysis of 40 ug whole cell extracts from HeLa cells transfected with either Ty1-tagged MBD1 (lanes 1 and 3) or Ty1-tagged MBD2 (lane 2 and 4). The WB was performed with the DYKDDDDK Epitope Tag Antibody (C.15200054) [NBP2-59146] diluted 1:1000 in TBS-Tween containing 5% skimmed milk (lane 1 and 2) or with an MBD1 antibody (lane 3) or an MBD2 antibody (lane 4). The position of the Ty1-tagged MBD1 and Ty1-tagged MBD2 are shown on the right, the marker (kDa) is shown on the left.
Chromatin Immunoprecipitation: DYKDDDDK Epitope Tag Antibody (C.15200054) [NBP2-59146] - Results obtained with the [NBP2-59146]. ChIP assays were performed using Plasmodium falciparium cells stably expressing Ty1-H2A (blue bars) and wild type Plasmodium falciparium cells (brown bars). Primer pairs were used for active genes, inactive genes, and variable genes. Sheared chromatin from 1x10^6 cells and 2 ug of antibody against Ty1 were used per ChIP experiment.

Applications for DYKDDDDK Epitope Tag Antibody (C.15200054) - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

2 ug/IP

Western Blot

1:1000 - 1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DYKDDDDK Epitope Tag

Fusion proteins, in which a known peptide such as a His-Tag, GST-Tag, or DYKDDDDK (FLAG(R)) is cloned with the target of interest, were developed to simplify protein isolation. Since specific antibodies may not be available against the protein of interest, epitope tag antibodies are commonly used to detect protein expression.

The epitope tag, DYKDDDDK, is comprised of an eight amino acid sequence derived from the gene-10 product from bacteriophage T7 and contains an enterokinase-cleavage site (1). Its hydrophilic nature and short peptide sequence reduce possible interference with protein expression, post-translational modifications, protein interactions, and protein function (2). The DYKDDDDK (FLAG(R)) epitope tag can either be fused to the N-terminus, C-terminus, or placed at an internal position of the target protein, facilitating characterization of the protein of interest using immunodetection methods. Compared to the standard DYKDDDDK epitope tag antibody, the rat monoclonal DYKDDDDK Epitope Tag antibody (clone L5) is 2-8 times more sensitive (3).

References

1. Brizzard BL, Chubet RG, Vizard DL. (1994) Immunoaffinity purification of (FLAG(R)) epitope-tagged bacterial alkaline phosphatase using a novel monoclonal antibody and peptide elution. Biotechniques. 16(4):730-5. PMID: 8024796

2. Einhauer A, Jungbauer A. (2001) The (FLAG(R)) peptide, a versatile fusion tag for the purification of recombinant proteins. J Biochem Biophys Methods. 49(1-3):455-65. PMID: 11694294

3. Park SH, Cheong C, Idoyaga J, Kim JY, Choi JH, Do Y, Lee H, Jo JH, Oh YS, Im W, Steinman RM, Park CG. (2008) Generation and application of new rat monoclonal antibodies against synthetic DYKDDDDK Epitope Tag (FLAG(R)) and OLLAS tags for improved immunodetection. J Immunol Methods. 331(1-2):27-38. PMID: 18054954

FLAG(R) is a trademark of Sigma-Aldrich.

Alternate Names

FLAG Tag

Additional DYKDDDDK Epitope Tag Products

Product Documents for DYKDDDDK Epitope Tag Antibody (C.15200054) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DYKDDDDK Epitope Tag Antibody (C.15200054) - BSA Free

FLAG(R) and ANTI-FLAG(R) are registered trademarks of Sigma-Aldrich Co. LLC.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for DYKDDDDK Epitope Tag Antibody (C.15200054) - BSA Free

Showing  1 - 5 of 6 FAQs Showing All

  • Q: Are the FLAG Tag antibodies validated in Simple Western?

    A: Yes, we offer 3 FLAG Tag antibodies that have been tested in Simple Western: NBP1-71705, NBP1-97410, and MAB8529.

  • Q: Do FLAG Tag antibodies comes in lyophilized form?

    A: The following FLAG Tag antibodies come in lyophilized form: NBP1-97393, NBP1-97401, NBP1-97399, NBP1-97400, and NBP1-97402.

  • Q: What is the immunogen sequence of this FLAG Tag antibody?

    A: The exact immunogen of DYKDDDDK Epitope Tag Antibody (C.15200054) is propietary information.

  • Q: What the theoretical molecular weight for FLAG Tag antibodies?

    A: The TMW for DYKDDDDK epitope FLAG Tag is approximately 1.01 kDa.

  • Q: Will FLAG Tag antibodies detect internal FLAG tags? My FLAG sequence is sandwiched between two other protein sequences.

    A: If you are applying your sample(s) to SDS-PAGE, under reducing condition and follow by Western Blot, DYKDDDDK epitope tag antibodies should be able to detect the tag region located internally. However, it depends on the property of your chimeric protein and if your tag is in the hydrophobic or hydrophilic region. Although DYKDDDDK is very hydrophilic, it may not be exposed to the surface if embedded deeply inside the protein with a high content of hydrophobic amino acids with or without covalently-linked modifications such as lipid moieties.

  • Q: Will FLAG Tag antibodies recognize FLAG at the C-terminal end?

    A: Yes, FLAG Tag antibodies can recognize FLAG-tag at both the N- or C-terminal.

  • Q: Are the FLAG Tag antibodies validated in Simple Western?

    A: Yes, we offer 3 FLAG Tag antibodies that have been tested in Simple Western: NBP1-71705, NBP1-97410, and MAB8529.

  • Q: Do FLAG Tag antibodies comes in lyophilized form?

    A: The following FLAG Tag antibodies come in lyophilized form: NBP1-97393, NBP1-97401, NBP1-97399, NBP1-97400, and NBP1-97402.

  • Q: What is the immunogen sequence of this FLAG Tag antibody?

    A: The exact immunogen of DYKDDDDK Epitope Tag Antibody (C.15200054) is propietary information.

  • Q: What the theoretical molecular weight for FLAG Tag antibodies?

    A: The TMW for DYKDDDDK epitope FLAG Tag is approximately 1.01 kDa.

  • Q: Will FLAG Tag antibodies detect internal FLAG tags? My FLAG sequence is sandwiched between two other protein sequences.

    A: If you are applying your sample(s) to SDS-PAGE, under reducing condition and follow by Western Blot, DYKDDDDK epitope tag antibodies should be able to detect the tag region located internally. However, it depends on the property of your chimeric protein and if your tag is in the hydrophobic or hydrophilic region. Although DYKDDDDK is very hydrophilic, it may not be exposed to the surface if embedded deeply inside the protein with a high content of hydrophobic amino acids with or without covalently-linked modifications such as lipid moieties.

  • Q: Will FLAG Tag antibodies recognize FLAG at the C-terminal end?

    A: Yes, FLAG Tag antibodies can recognize FLAG-tag at both the N- or C-terminal.

  • Q: Are the FLAG Tag antibodies validated in Simple Western?

    A: Yes, we offer 3 FLAG Tag antibodies that have been tested in Simple Western: NBP1-71705, NBP1-97410, and MAB8529.

  • Q: Do FLAG Tag antibodies comes in lyophilized form?

    A: The following FLAG Tag antibodies come in lyophilized form: NBP1-97393, NBP1-97401, NBP1-97399, NBP1-97400, and NBP1-97402.

  • Q: What is the immunogen sequence of this FLAG Tag antibody?

    A: The exact immunogen of DYKDDDDK Epitope Tag Antibody (C.15200054) is propietary information.

  • Q: What the theoretical molecular weight for FLAG Tag antibodies?

    A: The TMW for DYKDDDDK epitope FLAG Tag is approximately 1.01 kDa.

  • Q: Will FLAG Tag antibodies detect internal FLAG tags? My FLAG sequence is sandwiched between two other protein sequences.

    A: If you are applying your sample(s) to SDS-PAGE, under reducing condition and follow by Western Blot, DYKDDDDK epitope tag antibodies should be able to detect the tag region located internally. However, it depends on the property of your chimeric protein and if your tag is in the hydrophobic or hydrophilic region. Although DYKDDDDK is very hydrophilic, it may not be exposed to the surface if embedded deeply inside the protein with a high content of hydrophobic amino acids with or without covalently-linked modifications such as lipid moieties.

  • Q: Will FLAG Tag antibodies recognize FLAG at the C-terminal end?

    A: Yes, FLAG Tag antibodies can recognize FLAG-tag at both the N- or C-terminal.

  • Q: Are the FLAG Tag antibodies validated in Simple Western?

    A: Yes, we offer 3 FLAG Tag antibodies that have been tested in Simple Western: NBP1-71705, NBP1-97410, and MAB8529.

  • Q: Do FLAG Tag antibodies comes in lyophilized form?

    A: The following FLAG Tag antibodies come in lyophilized form: NBP1-97393, NBP1-97401, NBP1-97399, NBP1-97400, and NBP1-97402.

  • Q: What is the immunogen sequence of this FLAG Tag antibody?

    A: The exact immunogen of DYKDDDDK Epitope Tag Antibody (C.15200054) is propietary information.

  • Q: What the theoretical molecular weight for FLAG Tag antibodies?

    A: The TMW for DYKDDDDK epitope FLAG Tag is approximately 1.01 kDa.

  • Q: Will FLAG Tag antibodies detect internal FLAG tags? My FLAG sequence is sandwiched between two other protein sequences.

    A: If you are applying your sample(s) to SDS-PAGE, under reducing condition and follow by Western Blot, DYKDDDDK epitope tag antibodies should be able to detect the tag region located internally. However, it depends on the property of your chimeric protein and if your tag is in the hydrophobic or hydrophilic region. Although DYKDDDDK is very hydrophilic, it may not be exposed to the surface if embedded deeply inside the protein with a high content of hydrophobic amino acids with or without covalently-linked modifications such as lipid moieties.

  • Q: Will FLAG Tag antibodies recognize FLAG at the C-terminal end?

    A: Yes, FLAG Tag antibodies can recognize FLAG-tag at both the N- or C-terminal.

  • Q: Are the FLAG Tag antibodies validated in Simple Western?

    A: Yes, we offer 3 FLAG Tag antibodies that have been tested in Simple Western: NBP1-71705, NBP1-97410, and MAB8529.

  • Q: Do FLAG Tag antibodies comes in lyophilized form?

    A: The following FLAG Tag antibodies come in lyophilized form: NBP1-97393, NBP1-97401, NBP1-97399, NBP1-97400, and NBP1-97402.

  • Q: What is the immunogen sequence of this FLAG Tag antibody?

    A: The exact immunogen of DYKDDDDK Epitope Tag Antibody (C.15200054) is propietary information.

  • Q: What the theoretical molecular weight for FLAG Tag antibodies?

    A: The TMW for DYKDDDDK epitope FLAG Tag is approximately 1.01 kDa.

  • Q: Will FLAG Tag antibodies detect internal FLAG tags? My FLAG sequence is sandwiched between two other protein sequences.

    A: If you are applying your sample(s) to SDS-PAGE, under reducing condition and follow by Western Blot, DYKDDDDK epitope tag antibodies should be able to detect the tag region located internally. However, it depends on the property of your chimeric protein and if your tag is in the hydrophobic or hydrophilic region. Although DYKDDDDK is very hydrophilic, it may not be exposed to the surface if embedded deeply inside the protein with a high content of hydrophobic amino acids with or without covalently-linked modifications such as lipid moieties.

  • Q: Will FLAG Tag antibodies recognize FLAG at the C-terminal end?

    A: Yes, FLAG Tag antibodies can recognize FLAG-tag at both the N- or C-terminal.

  • Q: Are the FLAG Tag antibodies validated in Simple Western?

    A: Yes, we offer 3 FLAG Tag antibodies that have been tested in Simple Western: NBP1-71705, NBP1-97410, and MAB8529.

  • Q: Do FLAG Tag antibodies comes in lyophilized form?

    A: The following FLAG Tag antibodies come in lyophilized form: NBP1-97393, NBP1-97401, NBP1-97399, NBP1-97400, and NBP1-97402.

  • Q: What is the immunogen sequence of this FLAG Tag antibody?

    A: The exact immunogen of DYKDDDDK Epitope Tag Antibody (C.15200054) is propietary information.

  • Q: What the theoretical molecular weight for FLAG Tag antibodies?

    A: The TMW for DYKDDDDK epitope FLAG Tag is approximately 1.01 kDa.

  • Q: Will FLAG Tag antibodies detect internal FLAG tags? My FLAG sequence is sandwiched between two other protein sequences.

    A: If you are applying your sample(s) to SDS-PAGE, under reducing condition and follow by Western Blot, DYKDDDDK epitope tag antibodies should be able to detect the tag region located internally. However, it depends on the property of your chimeric protein and if your tag is in the hydrophobic or hydrophilic region. Although DYKDDDDK is very hydrophilic, it may not be exposed to the surface if embedded deeply inside the protein with a high content of hydrophobic amino acids with or without covalently-linked modifications such as lipid moieties.

  • Q: Will FLAG Tag antibodies recognize FLAG at the C-terminal end?

    A: Yes, FLAG Tag antibodies can recognize FLAG-tag at both the N- or C-terminal.

Showing  1 - 5 of 6 FAQs Showing All
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