Catalog Number: 5208
Chemical Name: (2S,3S)-3-[[[(1S)-1-[[[4-(Aminoiminomethyl)amino]butyl]amino]carbonyl]-3-methylbutyl]amino]carbonyl]-2-oxiranecarboxylic acid
Biological Activity
Potent and irreversible cysteine protease inhibitor (IC50 values are 1.4, 2.5 and 4.1 nM for cathepsin K, L and S respectively. Also inhibits papain, calpain, cathepsin B and cathepsin H. Has no effect on serine proteases. Reduces HSC-3 tongue cancer cell invasion and induces oxidative stress and apoptosis in filarial parasites. Also inhibits autophagy-associated lysosomal degradation in vitro.
Technical Data
  • M.Wt:
    357.41
  • Formula:
    C15H27N5O5
  • Solubility:
    Soluble to 20 mM in water
  • Purity:
    >98%
  • Storage:
    Store at -20°C
  • CAS No:
    66701-25-5
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Background References
  1. Human primary osteoclasts: in vitro generation and applications as pharmacological and clinical assay.
    Susa et al.
    J.Transl.Med., 2004;2:6
  2. Cathepsin K is present in invasive oral tongue squamous cell carcinoma in vivo and in vitro.
    Bitu et al.
    PLoS ONE, 2013;8:e70925
  3. Inhibition of cathepsin B by E-64 induces oxidative stress and apoptosis in filarial parasite.
    Wadhawan et al.
    PLoS ONE, 2014;9:e93161
  4. Cytosolic FoxO1 is essential for the induction of autophagy and tumour suppressor activity.
    Zhao et al.
    Nat.Cell.Biol., 2010;12:665
Citations:

The citations listed below are publications that use Tocris products. Selected citations for E 64 include:

1 Citations: Showing 1 - 1

  1. Tricyclic Antidepressants Amitriptyline and Desipramine Induced Neurotoxicity Associated with Parkinson's Disease.
    Authors: Lee Et al.
    PLoS One 2015;38:734

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