Eg5 Antibody (6G8V8)

Novus Biologicals | Catalog # NBP3-16458

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6G8V8 expressed in HEK293
View all Eg5 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 900-1056 of human Eg5 (P52732). ETIKIGLTKLNCFLEQDLKLDIPTGTTPQRKSYLYPSTLVRTEPREHLLDQLKRKQPELLMMLNCSENNKEETIPDVDVEEAVLGQYTEEPLSQEPSVDAGVDCSSIGGVPFFQHKKSHGKDKENRGINTLERSKVEETTEHLVTKSRLPLRAQINL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Eg5 Antibody (6G8V8)

Western Blot: Eg5 Antibody (6G8V8) [NBP3-16458]

Western Blot: Eg5 Antibody (6G8V8) [NBP3-16458]

Western Blot: Eg5 Antibody (6G8V8) [NBP3-16458] - Western blot analysis of extracts of various cell lines, using Eg5 Rabbit mAb (NBP3-16458) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Applications for Eg5 Antibody (6G8V8)

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Eg5

Eg5 is a mitotic motor protein required for chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis. Eg-5 is therefore crucial for proper chromosome segregation during cell division. Eg 5 antibodies are useful tools for cell cycle studies and research on mitotic spindles.

Long Name

Kinesin-like protein KIF11

Alternate Names

KIF11, KNSL1, TRIP-5, TRIP5

Gene Symbol

KIF11

Additional Eg5 Products

Product Documents for Eg5 Antibody (6G8V8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Eg5 Antibody (6G8V8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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