eGFP Antibody (5F3)
Novus Biologicals | Catalog # NBP3-48402
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Non-species specific
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5F3
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Product Specifications
Immunogen
The exact amino acid sequence is proprietary.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This product is shipped at ambient temperature. Store at -20C to -80C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80C (Avoid repeated freezing and thawing).
Scientific Data Images for eGFP Antibody (5F3)
Western Blot: eGFP Antibody (5F3) [NBP3-48402]
WB examination of Rabbit anti-EGFP mAb. 293T were transfected with or without EGFP and cell lysates were subjected to WB examination. Cells expressing EGFP show clear positive band at correct position.Applications for eGFP Antibody (5F3)
Application
Recommended Usage
Immunoprecipitation
1:200
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified from cell culture supernatant
Reconstitution
Dissolve the lyophilized antibody in sterile and low endotoxin water. The recommend volume of water is lot specific. Please refer to the instructions printed on the product vial or contact technical support.
Formulation
PBS, pH 7.4, 5-8% Trehalose
Preservative
Trehalose
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C in powder form. Store at -80C once reconstituted.
Calculators
Background: eGFP
Alternate Names
Enhanced Green Fluorescent Protein, GFP, Green Fluorescent Protein
Additional eGFP Products
Product Documents for eGFP Antibody (5F3)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for eGFP Antibody (5F3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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