Recombinant eGFP Protein

Novus Biologicals | Catalog # NBP2-34923

Novus Biologicals
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Key Product Details

Source

E. coli

Accession Number

P42212

Applications

Western Blot, SDS-PAGE
View all eGFP Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 239 amino acids corresponding to eGFP Source: E. coli

Uniprot ID: P42212

Amino Acid Sequence: MVSKGEELFT GVVPILVELD GDVNGHKFSV SGEGEGDATY GKLTLKFICT TGKLPVPWPT LVTTLTYGVQ CFSRYPDHMK QHDFFKSAMP EGYVQERTIF FKDDGNYKTR AEVKFEGDTL VNRIELKGID FKEDGNILGH KLEYNYNSHN VYIMADKQKN GIKVNFKIRH NIEDGSVQLA DHYQQNTPIG DGPVLLPDNH YLSTQSALSK DPNEKRDHMV LLEFVTAAGI TLGMDELYK

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of eGFP as determined by LAL method.

Predicted Molecular Mass

26.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Use in WB reported in scientific literature (PMID:10.1007/s00299-021-02700-w)

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant eGFP Protein

SDS-PAGE: Recombinant eGFP Protein [NBP2-34923]

SDS-PAGE: Recombinant eGFP Protein [NBP2-34923]

SDS-Page: Human GFP Protein [NBP2-34923] - GFP Protein [NBP2-34923]

Formulation, Preparation, and Storage

NBP2-34923
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: eGFP

Green fluorescent protein (GFP) here refers to the protein first purified from jellyfish Aequorea victoria, though many other organisms have similar proteins. It is a 26.9 kDa protein (composed of 238 a.a. residues) that shows green fluorescence in shortwave light (blue to ultraviolet). Despite of wild-type GFP, many mutants of GFP have been engineered for wider usage in research. Enhanced GFP (eGFP) has S65T and F64L mutations, which make GFP show increased fluorescence and fold more efficiently under 37 degrees C, respectively. eGFP allows the use of GFP in mammalian cells. In A. Victoria, GFP plays roles as an energy transfer acceptor. It has long been used in cell and molecular biology as a reporter of gene expression. GFP can also been applied as a molecular thermometer to measure temperature accurately in fluids.

Alternate Names

Enhanced Green Fluorescent Protein, GFP, Green Fluorescent Protein

UniProt

P42212

Additional eGFP Products

Product Documents for Recombinant eGFP Protein

Certificate of Analysis

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Product Specific Notices for Recombinant eGFP Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Citations for Recombinant eGFP Protein

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