EMMPRIN/CD147 Antibody (MEM-M6/2) [FITC]

Novus Biologicals | Catalog # NBP1-44585

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Porcine

Applications

Flow Cytometry

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # MEM-M6/2
View all EMMPRIN/CD147 Primary Antibodies »

Product Specifications

Immunogen

A soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the entire extracellular region of CD147 fused to the DNA coding for the hinge region, CH2 and CH3 domain of human IgG1.

Epitope

This antibody recognises extracellular Ig domain D1 of CD147 antigen.

Localization

Cell Membrane

Specificity

The antibody MEM-M6/2 recognizes extracellular Ig domain D1 of CD147 (Neurothelin), a 50-60 kDa type I transmembrane glycoprotein primarily expressed on all leukocytes, red blood cells, platelets and endothelial cells; it is not expressed by resting lymphocytes. The antibody MEM-M6/1 is a high-affinity antibody capable of binding to unstimulated peripheral blood T cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Store in the dark. Avoid prolonged exposure to light.

Scientific Data Images for EMMPRIN/CD147 Antibody (MEM-M6/2) [FITC]

Flow Cytometry: EMMPRIN/CD147 Antibody (MEM-M6/2) [FITC] [NBP1-44585] - Surface staining of human peripheral blood with anti-human CD147 (MEM-M6/1) FITC.
Flow Cytometry: EMMPRIN/CD147 Antibody (MEM-M6/2) [FITC] [NBP1-44585] - Surface staining of human peripheral blood with anti-human CD147 (MEM-M6/1) biotin.

Applications for EMMPRIN/CD147 Antibody (MEM-M6/2) [FITC]

Application
Recommended Usage

Flow Cytometry

10 ug/ml
Application Notes
The reagent is designed for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul of whole blood or 10^6 cells in a suspension.

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Size Exclusion Chromatography

Formulation

Phosphate buffered saline (PBS), pH 7.4

Preservative

15mM Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: EMMPRIN/CD147

CD147 (basigin, neurothelin, OX-47, 5A11, CE9, M6) also known as EMMPRIN (extracellular matrix metalloproteinase inducer) or TCSF (tumour cell-derived collagenase-stimulatory factor) is an ubiquitously expressed cell surface protein with multiple glycosylated forms. The highest level of CD147 expression is on metabolically active cells, such as lymphoblasts, inflammatory cells, brown adipocytes and malignant tumour cells. CD147 has multiple functions, including facilitating of cell surface expression of monocarboxylate transporter proteins and extracellular matrix metalloproteinases, regulation of integrin functions, it plays roles in cell development and activation, fetal development or retinal function.

Long Name

Extracellular Matrix Metalloproteinase Inducer

Alternate Names

Basigin, BSG, CD147

Gene Symbol

BSG

Additional EMMPRIN/CD147 Products

Product Documents for EMMPRIN/CD147 Antibody (MEM-M6/2) [FITC]

Certificate of Analysis

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Product Specific Notices for EMMPRIN/CD147 Antibody (MEM-M6/2) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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