EMMPRIN/CD147 Antibody (MEM-M6/2) - BSA Free

Novus Biologicals | Catalog # NB500-561

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Porcine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Flow (Cell Surface), CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # MEM-M6/2

Format

BSA Free
View all EMMPRIN/CD147 Primary Antibodies »

Product Specifications

Immunogen

A soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the entire extracellular region of CD147 fused to the DNA coding for the hinge region, CH2 and CH3 domain of human IgG1.

Epitope

This antibody recognises extracellular Ig domain D1 of CD147 antigen.

Localization

Cell Membrane

Specificity

The antibody MEM-M6/2 recognizes extracellular Ig domain D1 of CD147 (Neurothelin), a 50-60 kDa type I transmembrane glycoprotein primarily expressed on all leukocytes, red blood cells, platelets and endothelial cells; it is not expressed by resting lymphocytes. The antibody MEM-M6/1 is a high-affinity antibody capable of binding to unstimulated peripheral blood T cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EMMPRIN/CD147 Antibody (MEM-M6/2) - BSA Free

Flow (Cell Surface): EMMPRIN/CD147 Antibody (MEM-M6/2) - BSA Free [NB500-561]

Flow (Cell Surface): EMMPRIN/CD147 Antibody (MEM-M6/2) - BSA Free [NB500-561]

Flow (Cell Surface): EMMPRIN/CD147 Antibody (MEM-M6/2) [NB500-561] - Flow Cytometry: EMMPRIN/CD147 Antibody (MEM-M6/2) [FITC] [NBP1-44585] - Surface staining of human peripheral blood with anti-human CD147 (MEM-M6/1) FITC. Image using the FITC form of this antibody.
Flow Cytometry: EMMPRIN/CD147 Antibody (MEM-M6/2) - BSA Free [NB500-561]

Flow Cytometry: EMMPRIN/CD147 Antibody (MEM-M6/2) - BSA Free [NB500-561]

Flow Cytometry: EMMPRIN/CD147 Antibody (MEM-M6/2) [NB500-561] - Flow Cytometry: EMMPRIN/CD147 Antibody (MEM-M6/2) [FITC] [NBP1-44585] - Surface staining of human peripheral blood with anti-human CD147 (MEM-M6/1) biotin. Image using the FITC form of this antibody.
EMMPRIN/CD147 Antibody (MEM-M6/2) - BSA Free Flow Cytometry: EMMPRIN/CD147 Antibody (MEM-M6/2) - BSA Free [NB500-561]

Flow Cytometry: EMMPRIN/CD147 Antibody (MEM-M6/2) - BSA Free [NB500-561]

Surface staining pattern of human peripheral whole blood stained using anti-human CD147 (MEM-M6/2) purified antibody (concentration in sample 0.6 ug/ml, GAM APC).

Applications for EMMPRIN/CD147 Antibody (MEM-M6/2) - BSA Free

Application
Recommended Usage

Flow Cytometry

4 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

10 ug/ml
Application Notes
This antibody is Cytof ready.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: EMMPRIN/CD147

CD147 plays a pivotal role in spermatogenesis, embryo implantation, neural network formation and tumor progression. It stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). CD147 may target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. CD147 also seems to be a receptor for oligomannosidic glycans. In vitro, it promotes outgrowth of astrocytic processes. CD147 forms homooligomers in a cis-dependent manner on the plasma membrane. It forms a complex with MMP1 at the tumor cell surface. CD147 interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. CD147 also interacts with ATP1B2, MAG and L1CAM.

Long Name

Extracellular Matrix Metalloproteinase Inducer

Alternate Names

Basigin, BSG, CD147

Gene Symbol

BSG

Additional EMMPRIN/CD147 Products

Product Documents for EMMPRIN/CD147 Antibody (MEM-M6/2) - BSA Free

Certificate of Analysis

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Product Specific Notices for EMMPRIN/CD147 Antibody (MEM-M6/2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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