Endoglycan/PODXL2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-30066

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Between 300 and 350

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Endoglycan/PODXL2 Antibody - BSA Free

Endoglycan/PODXL2 Antibody

Immunoprecipitation:Endoglycan/PODXL2 AntibodyNBP3-30066] -

Immunoprecipitation:Endoglycan/PODXL2 AntibodyNBP3-30066] -Detection of human Endoglycan by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Endoglycan antibody NBP3-30066 (lot NBP3-30066-1) used for IP at 6 µg per reaction. Endoglycan was also immunoprecipitated by rabbit anti-Endoglycan antibody. For blotting immunoprecipitated Endoglycan, NBP3-30066 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for Endoglycan/PODXL2 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate buffer, pH 7 to 8

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Endoglycan/PODXL2

Endoglycan, also named Podocalyxin-like 2 protein (PODLX2), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural and sequence similarity. It is expressed on hematopoietic precursors, endothelial cells, smooth muscle cells and leukocyte subpopulations. Endoglycan is a potential marker for hematopoietic progenitors. It also functions as an L-Selectin ligand and may be involved in the inhibition of stem cell differentiation and leukocyte-endothelial cell adhesion.

Alternate Names

PODLX2, PODXL2

Gene Symbol

PODXL2

Additional Endoglycan/PODXL2 Products

Product Documents for Endoglycan/PODXL2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Endoglycan/PODXL2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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