EOGT/AER61 Antibody (JE64-87)

Novus Biologicals | Catalog # NBP3-32007

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE64-87 expressed in HEK293
View all EOGT Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within Human EOGT/AER61 aa 1-200 / 527. (Uniprot: Q5NDL2)

Localization

Endoplasmic reticulum lumen.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EOGT/AER61 Antibody (JE64-87)

EOGT/AER61 Antibody (JE64-87)

Western Blot: EOGT/AER61 Antibody (JE64-87) [NBP3-32007]

Western Blot: EOGT/AER61 Antibody (JE64-87) [NBP3-32007] - Western blot analysis of EOGT/AER61 on different lysates with Rabbit anti-EOGT/AER61 antibody (NBP3-32007) at 1/2,000 dilution.

Lane 1: Neuro-2a cell lysate (20 ug/Lane)
Lane 2: U-87 MG cell lysate (20 ug/Lane)
Lane 3: HeLa cell lysate (20 ug/Lane)
Lane 4: Caco-2 cell lysate (20 ug/Lane)
Lane 5: HDLM-2 cell lysate (20 ug/Lane)
Lane 6: HepG2 cell lysate (20 ug/Lane)
Lane 7: Mouse brain tissue lysate (40 ug/Lane)

Predicted band size: 62 kDa
Observed band size: 62 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32007) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for EOGT/AER61 Antibody (JE64-87)

Application
Recommended Usage

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EOGT

Long Name

EGF Domain-specific O-linked N-acetylglucosamine (GlcNAc) Transferase

Alternate Names

AER61, C3orf64

Gene Symbol

EOGT

Additional EOGT Products

Product Documents for EOGT/AER61 Antibody (JE64-87)

Certificate of Analysis

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Product Specific Notices for EOGT/AER61 Antibody (JE64-87)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Glycobiology-related Enzymes

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