EOGT/AER61 Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-05987
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human EOGT/AER61 fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for EOGT/AER61 Antibody - BSA Free
Western Blot: EOGT/AER61 Antibody [NBP3-05987]
Western Blot: EOGT/AER61 Antibody [NBP3-05987] - EOGT/AER61 Antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.Secondary: Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:62 kDaObserved band size:62 kDaImmunoprecipitation: EOGT/AER61 Antibody [NBP3-05987]
Immunoprecipitation: EOGT/AER61 Antibody [NBP3-05987] - EGOT/AER61 antibody was immunoprecipitated using:Lane A: 0.5 mg HeLa Whole Cell Lysate4 uL EGOT/AER61 antibody and 60 ug of Immunomagnetic beads Protein AG.Primary antibody: EGOT/AER61 antibody, at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 62 kDaObserved band size: 62 kDaApplications for EOGT/AER61 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
1-5ul/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Format
BSA Free
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: EOGT
Long Name
EGF Domain-specific O-linked N-acetylglucosamine (GlcNAc) Transferase
Alternate Names
AER61, C3orf64
Gene Symbol
EOGT
Additional EOGT Products
Product Documents for EOGT/AER61 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for EOGT/AER61 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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