Human Ephrin-A5 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-82488

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.09 ng/mL

Assay Range

0.16 - 10 ng/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human Ephrin-A5 in samples. No Significant cross-reactivity or interference between Human Ephrin-A5 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 4.52

Inter-Assay Precision (Precision between assays) CV% 4.91

Recovery for Human Ephrin-A5 ELISA Kit (Colorimetric)

Recovery

90-109%

Linearity

Human Ephrin-A5 ELISA Kit (Colorimetric)

Scientific Data Images for Human Ephrin-A5 ELISA Kit (Colorimetric)

Human Ephrin-A5 ELISA Kit (Colorimetric)

ELISA: Human Ephrin-A5 ELISA Kit (Colorimetric) [NBP2-82488] -

ELISA: Human Ephrin-A5 ELISA Kit (Colorimetric) [NBP2-82488] - Standard Curve Reference

Kit Contents for Human Ephrin-A5 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Ephrin-A5

Ephrin A5, a member of the ephrin family, prevents axon bundling in cocultures of cortical neurons with astrocytes, a model of late stage nervous system development and differentiation. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. EPH receptors typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin ligands and receptors have been named by the Eph Nomenclature Committee (1997). Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are similarly divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.

Alternate Names

AL-1, EFL-5, EFNA5, EphrinA5, LERK-7, RAGS

Gene Symbol

EFNA5

Additional Ephrin-A5 Products

Product Documents for Human Ephrin-A5 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human Ephrin-A5 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Ephrin Ligands and Eph Receptors

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