Flotillin-1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16508

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Hamster

Predicted:

Bovine (100%), Chimpanzee (100%), Porcine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Simple Western, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Flotillin-1 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Flotillin-1. The exact sequence is proprietary.

Localization

Cell membrane; Peripheral membrane protein; Membrane; caveola; Peripheral membrane protein; Melanosome

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Flotillin-1 Antibody - BSA Free

Immunoprecipitation: Flotillin-1 Antibody [NBP2-16508]

Immunoprecipitation: Flotillin-1 Antibody [NBP2-16508]

HCT-116 whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of Flotillin 1 protein by 4 ug Flotillin 1 antibody [C3], C-term 10 % SDS-PAGE The immunoprecipitated Flotillin 1 protein was detected by Flotillin 1 antibody [C3], C-term diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Flotillin-1 Antibody

Western Blot: Flotillin-1 Antibody [NBP2-16508] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 1 antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Flotillin-1 Antibody - BSA Free

Western Blot-Flotillin-1 Antibody - BSA Free

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 1 antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Flotillin-1 Antibody - BSA Free

Western Blot: Flotillin-1 Antibody [NBP2-16508] -

Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 1 antibody [C3], C-term (NBP2-16508) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Flotillin-1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1:100-1:500

Simple Western

1:20

Western Blot

1:500-1:3000
Application Notes
See Simple Western Antibody Database for Simple Western validation: Extracted HER2+ Breast Cancer Donor FFPE and Healthy Donor FFPE as samples; separated by size; antibody dilution of 1:20; apparent MW was 113, 139 kDa

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Flotillin-1

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined. [provided by RefSeq]

Alternate Names

flotillin 1, flotillin-1, integral membrane component of caveolae

Gene Symbol

FLOT1

UniProt

O75955 (Human)

Additional Flotillin-1 Products

Product Documents for Flotillin-1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Flotillin-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Customer Reviews for Flotillin-1 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review Flotillin-1 Antibody - BSA Free and earn rewards!

Have you used Flotillin-1 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies