Flumazenil

Tocris Bioscience | Catalog # 1328

Benzodiazepine antagonist
Tocris Bioscience

Key Product Details

Description

Benzodiazepine antagonist

Alternative Names

Ro 15-1788
View all GABA-A Receptor Benzodiazepines »

Product Description

Flumazenil is a benzodiazepine antagonist, non-selective for α1, α2, α3 or α5-containing GABAA receptors. Centrally active upon systemic administration in vivo. Also inhibits repressor element-1 silencing transcription factor (REST) by targeting degradation.

Product Specifications for Flumazenil

Molecular Weight

303.29

Formula

C15H14FN3O3

Storage

Store at +4°C

Purity

≥99% (HPLC)

Chemical Name

8-Fluoro-5,6-dihydro-5-methyl-6-oxo-4H-imidazo[1,5-a][1,4]benzodiazepine-3-carboxylic acid, ethyl ester

CAS Number

78755-81-4

PubChem ID

3373

InChI Key

OFBIFZUFASYYRE-UHFFFAOYSA-N

SMILES

CCOC(=O)C1=C2CN(C)C(=O)C3=CC(F)=CC=C3N2C=N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 25

Preparing Stock Solutions for Flumazenil

The following data is based on the product molecular weight 303.29.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.25 mM 13.19 mL 65.94 mL 131.89 mL
1.25 mM 2.64 mL 13.19 mL 26.38 mL
2.5 mM 1.32 mL 6.59 mL 13.19 mL
12.5 mM 0.26 mL 1.32 mL 2.64 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 42 publications citing the usage of this product.

Product Documents for Flumazenil

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Flumazenil

For research use only

Related Research Areas

GABA Receptors

Customer Reviews for Flumazenil (2)

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  • Antagonism of Flumazenil to effects of Phenazepam
    Name: Anonymous
    Species: Prussian carp
    Assay Type: In Vitro
    Verified Customer | Posted 04/14/2020
    Soluble in DMSO, but requires sonication.
    Flumazenil (FMZ) was used to block action of other benzodiazepine ligand of GABA-A receptor (Phenazepam, PNZ) on decay kinetics of spontaneous inhibitory currents recorded from brain neurons of Prussian carp (Carassius gibelio). FMZ demonstrated competitive antagonism to PNZ in this research, both in vivo (behavioral tests) and in vitro (whole-cell voltage-clamp recordings, see example illustration).
    Flumazenil 1328
  • Flumazenil and in vivo chloride signals
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vivo
    Verified Customer | Posted 05/23/2019
    injected 15mg/kg i.p at a concentration of.8mg/ml in 5% DMSO in saline
    used with fiber photometry to validate a chloride sensor in vivo. response to footshock was measured at baseline and then at 15 min intervals post injection
    Flumazenil 1328

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