Recombinant Rat G-CSF Protein

Novus Biologicals | Catalog # NBP2-35201

Novus Biologicals
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Key Product Details

Source

E. coli

Applications

Bioactivity, SDS-PAGE
View all G-CSF Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 195 amino acids corresponding to G-CSF Source: E. coli

Uniprot ID: P97712

Amino Acid Sequence: KKIPLLTVSS LPPSLPLPRS FLLKSLEQVR KIQARNTELL EQLCATYKLC HPEELVLFGH SLGIPKASLS SCSSQALQQT KCLSQLHSGL FLYQGLLQAL AGISSELAPT LDMLHLDVDN FATTIWQQME SLGVAPTVQP TQSTMPIFTS AFQRRAGGVL VTSYLQSFLE TAHHALHHLP RPAQKHFPES LFISI

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of G-CSF as determined by LAL method.

Predicted Molecular Mass

21.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

G-CSF protein is fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using murine NFS-60 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 x 10^7 IU/mg

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rat G-CSF Protein

SDS-PAGE: Recombinant Rat G-CSF Protein [NBP2-35201]

SDS-PAGE: Recombinant Rat G-CSF Protein [NBP2-35201]

SDS-Page: Rat G-CSF Protein [NBP2-35201]

Formulation, Preparation, and Storage

NBP2-35201
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 5 mM Sodium Citrate, pH 4.0.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: G-CSF

Granulocyte colony stimulating factor (G-CSF) is a pleiotropic cytokine. It is mainly produced by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Besides, many other cell types can secreted this protein after LPS, IL-1 or TNF- alpha activation, which are fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells. Various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. G-CSF is cytokine that acts in hematopoiesis by controlling the production, differentiation and function of 2 related white cell populations of the blood, the granulocytes and the monocytesmacrophages. In addition, it may function in some adhesion or recognition events at the cell surface.

Long Name

Granulocyte Colony Stimulating Factor

Alternate Names

C17orf33, CSF3, CSF3OS, Filgrastim, GCSF, Lenograstim, Pluripoietin

Gene Symbol

CSF3

Additional G-CSF Products

Product Documents for Recombinant Rat G-CSF Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Rat G-CSF Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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