G gamma3 Antibody

Novus Biologicals | Catalog # NBP3-32398

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all G gamma3 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within human G gamma3 aa 25-75. (Uniprot: P63215)

Localization

Cell membrane.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for G gamma3 Antibody

G gamma3 Antibody

Western Blot: G gamma3 Antibody [NBP3-32398]

Western Blot: G gamma3 Antibody [NBP3-32398] - Western blot analysis of G gamma3 on different lysates with Rabbit anti-G gamma3 antibody (NBP3-32398) at 1/500 dilution.

Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 8 kDa
Observed band size: 11 kDa

Exposure time: 2 minutes;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32398) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Applications for G gamma3 Antibody

Application
Recommended Usage

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: G gamma3

G proteins are heterotrimers of alpha, beta, and gamma subunits. Gamma subunits, such as GNG3, contribute to thespecificity of the hundreds of receptor signaling pathways involving G proteins (Schwindinger et al., 2004 (PubMed15314181)).(supplied by OMIM)

Alternate Names

GNGT3, guanine nucleotide binding protein (G protein), gamma 3, guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-3, guanine nucleotide-binding protein gamma-3 subunit, NBP gamma-3

Gene Symbol

GNG3

Additional G gamma3 Products

Product Documents for G gamma3 Antibody

Certificate of Analysis

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Download SDS

Product Specific Notices for G gamma3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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