gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-30552

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all gamma-Glutamylcyclotransferase/CRF21 Primary Antibodies »

Product Specifications

Immunogen

Between 138 and 188

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody - BSA Free

gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody

Immunoprecipitation:gamma-Glutamylcyclotransferase/CRF21/GGCT AntibodyNBP3-30552] -

Immunoprecipitation:gamma-Glutamylcyclotransferase/CRF21/GGCT AntibodyNBP3-30552] -Detection of human GGCT by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GGCT antibody NBP3-30552 (lot NBP3-30552-1) used for IP at 6 µg per reaction. For blotting immunoprecipitated GGCT, NBP3-30552 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: gamma-Glutamylcyclotransferase/CRF21

CRF21 (Cytochrome c-releasing factor 21; also C7orf24 or LOC79017) is a 21 kDa AIG2-like family member. It is a cytosolic protein that induces the release of Cytochrome c from mitochondria, possibly initiating apoptosis. Human CRF21 is 188 amino acids (aa) in length and is a complex of beta-sheets and alpha-helices that create two terminal domains. It has been proposed that CRF21 forms homodimers. There are at least two possible splice variants of human CRF21, one showing a 70 aa substitution, while the second variant shows an 18 aa substitution for aa 97-188. Full-length human CRF21 is 82% aa identical to mouse CRF21.

Long Name

Cytochrome c Releasing Factor 21

Alternate Names

C7orf24, CRF21, gammaGlutamylcyclotransferase, GCTG, GGC, GGCT, LOC79017

Gene Symbol

GGCT

Additional gamma-Glutamylcyclotransferase/CRF21 Products

Product Documents for gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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