Glutathione Reductase Antibody (JE31-42)

Novus Biologicals | Catalog # NBP3-32391

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE31-42 expressed in HEK293
View all Glutathione Reductase Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human Glutathione Reductase aa 473-522 / 522. (Uniprot: P00390)

Localization

Mitochondrion; Cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glutathione Reductase Antibody (JE31-42)

Glutathione Reductase Antibody (JE31-42)

Western Blot: Glutathione Reductase Antibody (JE31-42) [NBP3-32391]

Western Blot: Glutathione Reductase Antibody (JE31-42) [NBP3-32391] - Western blot analysis of Glutathione Reductase on different lysates with Rabbit anti-Glutathione Reductase antibody (NBP3-32391) at 1/2,000 dilution.

Lane 1: HepG2 cell lysate (20 ug/Lane)
Lane 2: A549 cell lysate (20 ug/Lane)
Lane 3: K-562 cell lysate (20 ug/Lane)
Lane 4: Jurkat cell lysate (20 ug/Lane)
Lane 5: Mouse liver tissue lysate (40 ug/Lane)
Lane 6: Mouse brain tissue lysate (40 ug/Lane)
Lane 7: Mouse kidney tissue lysate (40 ug/Lane)
Lane 8: Rat lung tissue lysate (40 ug/Lane)
Lane 9: Rat kidney tissue lysate (40 ug/Lane)

Predicted band size: 56 kDa
Observed band size: 56 kDa

Exposure time: 10 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32391) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for Glutathione Reductase Antibody (JE31-42)

Application
Recommended Usage

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glutathione Reductase

Glutathione reductase (GR) is a member of pyridine nucleotide-disulfideoxidoreductases, which includes the closely related enzymes thioredoxin reductase, lipoamide dehydrogenase, trypanothione reductase and mercuric ion reductase. GR is a cytoplasmic flavoenzyme widely distributed in aerobic organisms. The dimeric protein is composed of two identical subunits, each containing 1 FAD and 1 redox-active disulfide/dithiol as components of the catalytic apparatus. It plays a role in maintaining glutathione(GSH) in its reduced form by catalyzing the reduction of glutathione disulfide (GSSG): GSSG + NADPH + H+ ->2GSH + NADP+. In mosteukaryotic cells, GR maintains the ratio of [GSH]/[GSSG], and participates in several vital functions such as the detoxification of reactive oxygen species as well as protein and DNA biosynthesis.

Alternate Names

GLUR, GRase, GRD1, GSR, HEL-752

Gene Symbol

GSR

Additional Glutathione Reductase Products

Product Documents for Glutathione Reductase Antibody (JE31-42)

Certificate of Analysis

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Product Specific Notices for Glutathione Reductase Antibody (JE31-42)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Redox Enzymes

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