Human Glutathione Reductase ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-82193

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant (100 uL)

Sensitivity

46.88 pg/mL

Assay Range

78.13 - 5000 pg/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human Glutathione Reductase in samples. No significant cross-reactivity or interference between Human Glutathione Reductase and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) %CV 6

Inter-Assay Precision (Precision between assays) %CV 6

Recovery for Human Glutathione Reductase ELISA Kit (Colorimetric)

Recovery

average = 97% for serum; average = 92% for EDTA plasma; average = 93% for cell culture media

Linearity

Human Glutathione Reductase ELISA Kit (Colorimetric)

Scientific Data Images for Human Glutathione Reductase ELISA Kit (Colorimetric)

Human Glutathione Reductase ELISA Kit (Colorimetric)

ELISA: Human Glutathione Reductase ELISA Kit (Colorimetric) [NBP2-82193] -

ELISA: Human Glutathione Reductase ELISA Kit (Colorimetric) [NBP2-82193] - Standard Curve Reference

Kit Contents for Human Glutathione Reductase ELISA Kit (Colorimetric)

  • Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Glutathione Reductase

Glutathione reductase (GR) is a member of pyridine nucleotide-disulfideoxidoreductases, which includes the closely related enzymes thioredoxin reductase, lipoamide dehydrogenase, trypanothione reductase and mercuric ion reductase. GR is a cytoplasmic flavoenzyme widely distributed in aerobic organisms. The dimeric protein is composed of two identical subunits, each containing 1 FAD and 1 redox-active disulfide/dithiol as components of the catalytic apparatus. It plays a role in maintaining glutathione(GSH) in its reduced form by catalyzing the reduction of glutathione disulfide (GSSG): GSSG + NADPH + H+ ->2GSH + NADP+. In mosteukaryotic cells, GR maintains the ratio of [GSH]/[GSSG], and participates in several vital functions such as the detoxification of reactive oxygen species as well as protein and DNA biosynthesis.

Alternate Names

GLUR, GRase, GRD1, GSR, HEL-752

Gene Symbol

GSR

Additional Glutathione Reductase Products

Product Documents for Human Glutathione Reductase ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human Glutathione Reductase ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Redox Enzymes

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