GPD2 Antibody (PSH0-31)

Western Blot: GPD2 Antibody (PSH0-31) [NBP3-32402]

Western Blot: GPD2 Antibody (PSH0-31) [NBP3-32402] - Western blot analysis of GPD2 on different lysates with Rabbit anti-GPD2 antibody (NBP3-32402) at 1/1,000 dilution.

Lane 1: MCF-7 cell lysate
Lane 2: THP-1 cell lysate
Lane 3: U87-MG cell lysate
Lane 4: HeLa cell lysate

Lysates/proteins at 10 ug/Lane.

Predicted band size: 81 kDa
Observed band size: 68 kDa

Exposure time: 10s;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32402) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Flow Cytometry: GPD2 Antibody (PSH0-31) [NBP3-32402]

Flow Cytometry: GPD2 Antibody (PSH0-31) [NBP3-32402] - Flow cytometric analysis of Hela cells labeling GPD2.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32402, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).