GR/NR3C1 Antibody (2K3E8)

Novus Biologicals | Catalog # NBP3-15789

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2K3E8 expressed in HEK293
View all GR/NR3C1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 2-180 of human GR (P04150). DSKESLTPGREENPSSVLAQERGDVMDFYKTLRGGATVKVSASSPSLAVASQSDSKQRRLLVDFPKGSVSNAQQPDLSKAVSLSMGLYMGETETKVMGNDLGFPQQGQISLSSGETDLKLLEESIANLNRSTSVPENPKSSASTAVSAAPTEKEFPKTHSDVSSEQQHLKGQTGTNGGN

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GR/NR3C1 Antibody (2K3E8)

Western Blot: GR/NR3C1 Antibody (2K3E8) [NBP3-15789]

Western Blot: GR/NR3C1 Antibody (2K3E8) [NBP3-15789]

Western Blot: GR/NR3C1 Antibody (2K3E8) [NBP3-15789] - Western blot analysis of extracts of various cell lines, using GR/NR3C1 antibody (NBP3-15789) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunoprecipitation: GR/NR3C1 Antibody (2K3E8) [NBP3-15789]

Immunoprecipitation: GR/NR3C1 Antibody (2K3E8) [NBP3-15789]

Immunoprecipitation: GR/NR3C1 Antibody (2K3E8) [NBP3-15789] - Immunoprecipitation analysis of 300ug extracts of U-251MG cells using 3ug GR/NR3C1 antibody (NBP3-15789). Western blot was performed from the immunoprecipitate using GR/NR3C1 antibody (NBP3-15789) at a dilition of 1:1000.

Applications for GR/NR3C1 Antibody (2K3E8)

Application
Recommended Usage

Immunoprecipitation

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GR/NR3C1

Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor. The corresponding gene for the glucocorticoid receptor is NR3C1.

Long Name

Glucocorticoid Receptor

Alternate Names

NR3C1

Gene Symbol

NR3C1

Additional GR/NR3C1 Products

Product Documents for GR/NR3C1 Antibody (2K3E8)

Certificate of Analysis

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Product Specific Notices for GR/NR3C1 Antibody (2K3E8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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Associated Pathways

TGF-beta Signaling Pathways TGF-beta Signaling Pathway Thumbnail