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Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Between 725 and 775
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HIP1 Antibody - BSA Free
Immunoprecipitation:HIP1 AntibodyNBP3-28977] -
Immunoprecipitation:HIP1 AntibodyNBP3-28977] -Detection of human HIP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HIP1 antibody NBP3-28977 (lot NBP3-28977-1) used for IP at 6 µg per reaction. HIP1 was also immunoprecipitated by rabbit anti-HIP1 antibody. For blotting immunoprecipitated HIP1, NBP3-28977 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for HIP1 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
Tris-citrate/phosphate pH 7 -8.
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: HIP1
In addition to playing a role in Huntingtin disease, HIP1 is likely to be involved in the recruitment of clathrin coats to lipid membranes, and it may also factor in tumorigenesis by allowing the survival of precancerous and cancerous cells. Since HIP-1 expression is significantly associated with prostate and colon cancer metastasis, HIP-1 can serve as a putative prognostic factor for prostate and colon cancers.
Alternate Names
HIP-1, HIP-I, huntingtin interacting protein 1, huntingtin-interacting protein 1, Huntingtin-interacting protein I, ILWEQ, MGC126506
Gene Symbol
HIP1
Additional HIP1 Products
Product Documents for HIP1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HIP1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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