Recombinant Human Histone H2AX His Protein

Novus Biologicals | Catalog # NBP1-42464

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

Western Blot, SDS-PAGE
View all Histone H2AX Proteins and Enzymes »

Product Specifications

Description

Recombinant H2AX protein corresponding to amino acids 2 - 143.

Source: E. coli

(Accession #: P16104)

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

17.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

This product is useful as a positive control for both Western Blot and SDS-Page. This protein has been tested using NB100-638 for detection on a Western Blot.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Histone H2AX His Protein

Western Blot: Recombinant Human Histone H2AX His Protein [NBP1-42464]

Western Blot: Recombinant Human Histone H2AX His Protein [NBP1-42464]

Western Blot: H2AX Protein [NBP1-42464] - The anti-H2AX antibody NB100-638 was used to detect this protein. 200ng of protein was loaded onto the gel and NB100-638 was used to detect the protein.
SDS-PAGE: Recombinant Human Histone H2AX His Protein [NBP1-42464]

SDS-PAGE: Recombinant Human Histone H2AX His Protein [NBP1-42464]

SDS-Page: H2AX Protein [NBP1-42464] - This H2AX protein was tested on a 4-20% SDS-PAGE gel with 5ug of protein loaded on the gel. The purity of the protein is >95%.

Formulation, Preparation, and Storage

NBP1-42464
Formulation 25 mM Tris pH 8.0, 250 mM NaCl, 8 M Urea, 1 mM DTT
Concentration 0.5 mg/ml
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -80C. Avoid freeze-thaw cycles.

Background: Histone H2AX

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. Human Histone H2AX with an N-terminal His tag expressed in E. coli and purified by conventional chromatography.

Alternate Names

H2AFX

Gene Symbol

H2AX

Additional Histone H2AX Products

Product Documents for Recombinant Human Histone H2AX His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human Histone H2AX His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

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