Horse IgG, Fab2 Native Protein

Novus Biologicals | Catalog # NBP1-96781

Novus Biologicals

Key Product Details

Applications

Western Blot, SDS-PAGE
View all Horse IgG, Fab2 Proteins and Enzymes »

Product Specifications

Description

Native Equine Horse IgG, Fab2

Source: Normal serum

Store vial at 4C upon receipt. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Purity

Multi-step

Applications

Western Blot (1:100-1:2000)

Application Notes

This product has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Protein / Peptide Type

Native Protein

Scientific Data Images for Horse IgG, Fab2 Native Protein

SDS-PAGE: Horse IgG, Fab2 Native Protein [NBP1-96781]

SDS-PAGE: Horse IgG, Fab2 Native Protein [NBP1-96781]

SDS-Page: Horse IgG, Fab2 Native Protein [NBP1-96781] - SDS-PAGE of Horse F(ab')2 Fragment. Lane 1: 1x Reduced, Horse F(ab')2 Fragment. Lane 2: Molecular Weight Ladder. 4-20% SDS-PAGE; Coomassie Stained.

Formulation, Preparation, and Storage

NBP1-96781
Preparation Method This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Horse IgG, anti-Horse IgG F(ab')2 and anti-Horse Serum. No reaction was observed against anti-Horse IgG F(c) or anti-Pepsin.
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative 0.01% Sodium Azide
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Horse IgG, Fab2

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.

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Product Documents for Horse IgG, Fab2 Native Protein

Certificate of Analysis

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Product Specific Notices for Horse IgG, Fab2 Native Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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