Human Carbonic Anhydrase VB/CA5B Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB3176U Datasheet / COA / SDS

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FAB3176U-100UG

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All Carbonic Anhydrase VB/CA5B Antibodies

Product Details

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Carbonic Anhydrase VB/CA5B Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Carbonic Anhydrase VB/CA5B in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CA1, 2, 3, 4, 5A, 6, 7, 8, or 9 is observed.

Source

Monoclonal Mouse IgG2b Clone # 491725

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human Carbonic Anhydrase VB/CA5B
Cys34-Pro317
Accession # Q9Y2D0

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Carbonic Anhydrase VB/CA5B

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6^th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO₂ removal and mosquito control in industry. Carbonic Anhydrase VB encoded by the CA5B gene is a mitochondrial protein. In comparison with another mitochondrial CA (CA5A), CA5B has different tissue distribution and chromosomal location (2, 3). The amino acid sequence of human CA5B (residues 34 to 317) is 94%, 93%, 92%, and 74% identical to that of canine, mouse, bovine/rat, and chicken.

Entrez Gene IDs

11238 (Human); 302669 (Rat)

Alternate Names

CA5B; Carbonate dehydratase VB; carbonic anhydrase 5B, mitochondrial; Carbonic Anhydrase VB; carbonic anhydrase VB, mitochondrial; carbonic dehydratase; CAVB; CA-VB; EC 4.2.1.1; MGC39962