Human CEACAM-18 APC‑conjugated Antibody

R&D Systems | Catalog # FAB9869A

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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 1057633
View all CEACAM-18 Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell, HEK293-derived human CEACAM-18
Gln31-His317
Accession # A8MTB9

Specificity

Detects human CEACAM-18 in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CEACAM-18 APC‑conjugated Antibody

Detection of CEACAM-18 in HEK293 cells by Flow Cytometry.

HEK293 cells transfected with Human CEACAM-18 (filled histogram) vs Irrelevant transfectant cells (open histogram) were stained with Mouse Anti-Human CEACAM-18 APC‑conjugated Monoclonal Antibody (Catalog # FAB9869A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CEACAM-18 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HEK293 cells transfected with Human CEACAM-18 vs Irrelevant transfectant cells

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CEACAM-18

Carcinoembryonic Antigen-related Cell Adhesion Molecule 18 (CEACAM-18) is part of the CEA protein family consisting of CEACAMs and the pregnancy-specific glycoproteins (PSGs). Both CEACAM and PSG molecules have been identified in humans and belong to the much larger glycosylphosphatidylinositol (GPI)-linked immunoglobulin (Ig) superfamily (1, 2). Mature human CEACAM-18 has a 298 amino acid (aa) extracellular domain containing 2 IgC2-like and 1 IgV-like domains, a single transmembrane domain and a short cytoplasmic tail (2). CEACAM-18 is one of only five conserved CEACAMs among mouse, rat, and human (2), but mature human CEACAM-18 has low aa sequence identify with mouse and rat at 60% and 58%, respectively. Originally discovered as a biomarker for colorectal cancer (3), CEACAMs have now been associated with numerous intracellular signaling processes including cell adhesion, cell growth, recognition and differentiation, angiogenesis, and apoptosis (4-6). While the exact function of CEACAM-18 has been yet to be elucidated, it may bind pathogen receptors or other immunoregulatory members (6). CEACAM family members were identified as the major Galectin-3 receptor candidates on human neutrophils (7). Binding of carbohydrate ligands to CEACAMs may be important in the release of proinflammatory mediators (8, 9).

References

  1. Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
  2. Zebhauser, R. et al. (2005) Genomics 86:566.
  3. Gold, P and Freedman SO, 1965) J Exp Med 122:467.
  4. Obrink, B. (1997) Curr Opin Cell Biol 9:616.
  5. Horst, A.K. and Wagener, C. (2004) Handb Exp Pharmacol 283.
  6. Kuespert, K. et al. (2006) Curr Opin Cell Biol. 18(5):565.
  7. Feuk-Lagerstedt, E. et al. (1999) J. Immunol. 163:5592.
  8. Yoon, J. et al. (2007) J. Immunol. 179:8454.
  9. Schröder, A.K. et al. (2006) Hum Immunol. 67:676.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 18

Alternate Names

Carcinoembryonic Antigen-Related Cell Adhesion Molecule 18

Entrez Gene IDs

729767 (Human); 72431 (Mouse); 502334 (Rat)

Gene Symbol

CEACAM18

UniProt

A8MTB9

Additional CEACAM-18 Products

Product Documents for Human CEACAM-18 APC‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human CEACAM-18 APC‑conjugated Antibody

For research use only

Related Research Areas

Immunoglobulin Superfamily CAMs

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