Mouse IgG1 APC-conjugated Antibody

(8 citations)
(2 Reviews)
  
  • Specificity
    Selected for use as mouse IgG1 isotype control for flow cytometry applications.
  • Source
    Monoclonal Mouse IgG1 kappa Clone # 11711
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    KLH
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Control
    Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse IgG Control by Flow Cytometry Human peripheral blood monocytes were stained with Mouse Anti-Human LILRA6/CD85b APC-conjugated Monoclonal Antibody (Catalog #FAB8656A,filled histogram) or Mouse IgG Isotype Control Antibody (Catalog # IC002A, open histogram). View our protocol for StainingMembrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG1

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G1
  • Entrez Gene IDs:
    101867415 (Cynomolgus Monkey)
  • Alternate Names:
    IgG1
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. Differential cytokine contributions of perivascular haematopoietic stem cell niches
    Authors: N Asada, Y Kunisaki, H Pierce, Z Wang, NF Fernandez, A Birbrair, A Ma'ayan, PS Frenette
    Nat. Cell Biol, 2017;19(3):214-223.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Biological Activity of Masked Endotoxin
    Authors: H Schwarz, J Gornicec, T Neuper, MA Parigiani, M Wallner, A Duschl, J Horejs-Hoe
    Sci Rep, 2017;7(0):44750.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow control
  3. Signaling via PI3K/FOXO1A pathway modulates formation and survival of human embryonic stem cell-derived endothelial cells.
    Authors: Merkely B, Gara E, Lendvai Z, Skopal J, Leja T, Zhou W, Kosztin A, Varady G, Mioulane M, Bagyura Z, Nemeth T, Harding S, Foldes G
    Stem Cells Dev, 2015;24(7):869-78.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Prerequisites for Functional Interleukin 31 Signaling and Its Feedback Regulation by Suppressor of Cytokine Signaling 3 (SOCS3).
    Authors: Maier E, Mittermeir M, Ess S, Neuper T, Schmiedlechner A, Duschl A, Horejs-Hoeck J
    J Biol Chem, 2015;290(41):24747-59.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. The Spiegelmer NOX-A12, a novel CXCL12 inhibitor, interferes with chronic lymphocytic leukemia cell motility and causes chemosensitization.
    Authors: Hoellenriegel J, Zboralski D, Maasch C, Rosin N, Wierda W, Keating M, Kruschinski A, Burger J
    Blood, 2014;123(7):1032-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  6. Cancer stem-like sphere cells induced from de-differentiated hepatocellular carcinoma-derived cell lines possess the resistance to anti-cancer drugs.
    Authors: Hashimoto, Noriaki, Tsunedomi, Ryouichi, Yoshimura, Kiyoshi, Watanabe, Yusaku, Hazama, Shoichi, Oka, Masaaki
    BMC Cancer, 2014;14(0):722.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow control
  7. VEGFR2 expressing circulating (progenitor) cell populations in volunteers and cancer patients.
    Authors: Vroling L, Yuana Y, Schuurhuis GJ, van Hinsbergh VW, Gundy C, de Haas R, van Cruijsen H, Boven E, Hoekman K, Broxterman HJ
    Thromb. Haemost., 2007;98(2):440-50.
    Species: Human
    Sample Type: Whole Blood
    Application: Ctrl
  8. Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration.
    Authors: Li Y, Fan J, Chen M, Li W, Woodley DT
    J. Invest. Dermatol., 2006;126(9):2096-105.
    Species: Human
    Sample Type: Serum
    Application: Ctrl
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

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Mouse IgG1 PE-conjugated Antibody

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Mouse IgG1 Fluorescein-conjugated Antibody

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Mouse IgG1 Alexa Fluor® 488-conjugated Antibody

Ctrl IC002G 3  
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Mouse IgG1 Alexa Fluor® 700-conjugated Antibody

Ctrl IC002N  
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Mouse IgG1 PerCP-conjugated Antibody

Ctrl IC002C  
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Mouse IgG1 Isotype Control

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Mouse IgG1 Biotinylated Antibody

Ctrl IC002B 2
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Mouse IgG1 Alexa Fluor® 647-conjugated Antibody

Ctrl IC002R
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Mouse IgG1 Alexa Fluor® 594-conjugated Antibody

Ctrl IC002T
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Mouse IgG1 Alexa Fluor® 750-conjugated Antibody

Ctrl IC002S
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Mouse IgG1 Alexa Fluor® 405-conjugated Antibody

Ctrl IC002V
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Average Rating: 4.5 (Based on 2 reviews)

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Read what people are saying who have used Mouse IgG1 APC-conjugated Antibody.

We have 2 reviews tested in 1 species: Human.
We have 2 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Mouse IgG1 APC-conjugated Antibody IC002A
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Excellent
 Flow Human Anonymous 09/10/2016
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Image Details
Flow Cytometry Mouse IgG1 APC-conjugated Antibody IC002A
Flow Cytometry: Mouse IgG1 APC-conjugated Antibody [IC002A]

Summary

ApplicationFlow Cytometry
Sample TestedASTROCYTE
SpeciesHuman
Flow Cytometry Mouse IgG1 APC-conjugated Antibody IC002A
Enlarge
Very Good
 Flow Human Anonymous 06/15/2016
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Image Details
Flow Cytometry Mouse IgG1 APC-conjugated Antibody IC002A
Flow Cytometry: Mouse IgG1 APC-conjugated Antibody [IC002A]

Summary

ApplicationFlow Cytometry
Sample TestedHuman embryonic stem cells derived mesoderm (H1)
SpeciesHuman

Other Experimental Details

Other Experimental Details1 million of H1 embryonic stem cells were incubated with 10ul of mouse IgG1 APC-conjugated and 10 ul of mouse IgG1 PE-conjugated Antibodies for 45min at 4C in dark. Cells were analyzed user the LSRII analyzer.

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