Mouse IgG1 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
IC002A
Detection of Mouse IgG Control by Flow Cytometry
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Product Details
Citations (12)
FAQs
Supplemental Products
Reviews (2)

Mouse IgG1 APC-conjugated Antibody Summary

Specificity
Selected for use as mouse IgG1 isotype control for flow cytometry applications.
Source
Monoclonal Mouse IgG1 kappa Clone # 11711
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Detection of Mouse IgG Control by Flow Cytometry Human peripheral blood monocytes were stained with Mouse Anti-Human LILRA6/CD85b APC-conjugated Monoclonal Antibody (Catalog #FAB8656A,filled histogram) or Mouse IgG Isotype Control Antibody (Catalog # IC002A, open histogram). View our protocol for StainingMembrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG1

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G1
Entrez Gene IDs
101867415 (Cynomolgus Monkey)
Alternate Names
IgG1

Product Datasheets

Citations for Mouse IgG1 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. CXCR7 expression in diffuse large B-cell lymphoma identifies a subgroup of CXCR4+ patients with good prognosis
    Authors: MJ Moreno, A Gallardo, S Novelli, A Mozos, M Aragó, MÁ Pavón, MV Céspedes, V Pallarès, A Falgàs, M Alcoceba, O Blanco, M Gonzalez-D, J Sierra, R Mangues, I Casanova
    PLoS ONE, 2018;13(6):e0198789.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  2. Regeneration of the lung alveolus by an evolutionarily conserved epithelial progenitor
    Authors: WJ Zacharias, DB Frank, JA Zepp, MP Morley, FA Alkhaleel, J Kong, S Zhou, E Cantu, EE Morrisey
    Nature, 2018;555(7695):251-255.
    Species: Human
    Sample Types: Whole Cells
    Applications: Ctrl
  3. Role of IL-18 in atopic asthma is determined by balance of IL-18/IL-18BP/IL-18R
    Authors: H Zhang, J Wang, L Wang, H Xie, L Chen, S He
    J. Cell. Mol. Med., 2017;0(0):.
    Applications: Flow Ctrl
  4. Differential cytokine contributions of perivascular haematopoietic stem cell niches
    Authors: N Asada, Y Kunisaki, H Pierce, Z Wang, NF Fernandez, A Birbrair, A Ma'ayan, PS Frenette
    Nat. Cell Biol, 2017;19(3):214-223.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  5. Biological Activity of Masked Endotoxin
    Authors: H Schwarz, J Gornicec, T Neuper, MA Parigiani, M Wallner, A Duschl, J Horejs-Hoe
    Sci Rep, 2017;7(0):44750.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow control
  6. Equine Mesenchymal Stromal Cells retain a pericyte-like phenotype
    Authors: CL Esteves, TA Sheldrake, L Dawson, T Menghini, E Rink, K Amilon, N Khan, B Peault, X Donadeu
    Stem Cells Dev., 2017;0(0):.
    Species: Equine
    Sample Types: Whole Cells
    Applications: Flow
  7. Signaling via PI3K/FOXO1A pathway modulates formation and survival of human embryonic stem cell-derived endothelial cells.
    Authors: Merkely B, Gara E, Lendvai Z, Skopal J, Leja T, Zhou W, Kosztin A, Varady G, Mioulane M, Bagyura Z, Nemeth T, Harding S, Foldes G
    Stem Cells Dev, 2015;24(7):869-78.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  8. Prerequisites for Functional Interleukin 31 Signaling and Its Feedback Regulation by Suppressor of Cytokine Signaling 3 (SOCS3).
    Authors: Maier E, Mittermeir M, Ess S, Neuper T, Schmiedlechner A, Duschl A, Horejs-Hoeck J
    J Biol Chem, 2015;290(41):24747-59.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  9. The Spiegelmer NOX-A12, a novel CXCL12 inhibitor, interferes with chronic lymphocytic leukemia cell motility and causes chemosensitization.
    Authors: Hoellenriegel J, Zboralski D, Maasch C, Rosin N, Wierda W, Keating M, Kruschinski A, Burger J
    Blood, 2014;123(7):1032-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  10. Cancer stem-like sphere cells induced from de-differentiated hepatocellular carcinoma-derived cell lines possess the resistance to anti-cancer drugs.
    Authors: Hashimoto, Noriaki, Tsunedomi, Ryouichi, Yoshimura, Kiyoshi, Watanabe, Yusaku, Hazama, Shoichi, Oka, Masaaki
    BMC Cancer, 2014;14(0):722.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow control
  11. VEGFR2 expressing circulating (progenitor) cell populations in volunteers and cancer patients.
    Authors: Vroling L, Yuana Y, Schuurhuis GJ, van Hinsbergh VW, Gundy C, de Haas R, van Cruijsen H, Boven E, Hoekman K, Broxterman HJ
    Thromb. Haemost., 2007;98(2):440-50.
    Species: Human
    Sample Types: Whole Blood
    Applications: Ctrl
  12. Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration.
    Authors: Li Y, Fan J, Chen M, Li W, Woodley DT
    J. Invest. Dermatol., 2006;126(9):2096-105.
    Species: Human
    Sample Types: Serum
    Applications: Ctrl

FAQs

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Reviews for Mouse IgG1 APC-conjugated Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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Mouse IgG1 APC-conjugated Antibody
By Anonymous on 09/10/2016
Application: Flow Sample Tested: ASTROCYTE Species: Human

Mouse IgG1 APC-conjugated Antibody
By Anonymous on 06/15/2016
Application: Flow Sample Tested: Human embryonic stem cells derived mesoderm (H1) Species: Human

1 million of H1 embryonic stem cells were incubated with 10ul of mouse IgG1 APC-conjugated and 10 ul of mouse IgG1 PE-conjugated Antibodies for 45min at 4C in dark. Cells were analyzed user the LSRII analyzer.