Mouse IgG1 APC-conjugated Antibody
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Summary
Data Examples
Preparation and Storage
Reconstitution Calculator
Background
Product Datasheets
Related Research Areas
Mouse IgG1 APC-conjugated Antibody Summary
Specificity
Selected for use as mouse IgG1 isotype control for flow cytometry applications.
Source
Monoclonal Mouse IgG1 kappa Clone # 11711
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)
Applications
Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Control
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Detection of Mouse IgG Control by Flow Cytometry Human peripheral blood monocytes were stained with Mouse Anti-Human LILRA6/CD85b APC-conjugated Monoclonal Antibody (Catalog # FAB8656A, filled histogram) or Mouse IgG Isotype Control Antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Reconstitution Calculator
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG1
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G1
Alternate Names
IgG1; Igh-4; RGD1359539; VH7183
Product Datasheets
Citations for Mouse IgG1 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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MET-induced CD73 restrains STING-mediated immunogenicity of EGFR-mutant lung cancer
Authors: R Yoshida, M Saigi, T Tani, BF Springer, H Shibata, S Kitajima, NR Mahadevan, M Campisi, W Kim, Y Kobayashi, TC Thai, K Haratani, Y Yamamoto, SK Sundararam, EH Knelson, A Vajdi, I Canadas, R Uppaluri, CP Paweletz, JJ Miret, PH Lizotte, PC Gokhale, PA Janne, DA Barbie
Cancer Research, 2022;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry Control -
Functional Downregulation of PD-L1 and PD-L2 by CpG and non-CpG Oligonucleotides in Melanoma Cells
Authors: J Kleemann, K Steinhorst, V König, N Zöller, J Cinatl, D Özistanbul, R Kaufmann, M Meissner, S Kippenberg
Cancers, 2022;14(19):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Nobiletin and Xanthohumol Sensitize Colorectal Cancer Stem Cells to Standard Chemotherapy
Authors: A Turdo, A Glaviano, G Pepe, F Calapà, S Raimondo, ME Fiori, D Carbone, MG Basilicata, V Di Sarno, C Ostacolo, B Parrino, S Cascioferr, C Pecoraro, S Di Franco, D Bellavia, M Gaggianesi, V Veschi, M Lo Iacono, G Ganduscio, VD Pantina, LR Mangiapane, MR Bongiorno, R Alessandro, M Todaro, R De Maria, P Diana, P Campiglia, G Stassi
Cancers, 2021;13(16):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Two 20-Residue-Long Peptides Derived from Plasmodium vivax Merozoite Surface Protein 10 EGF-Like Domains Are Involved in Binding to Human Reticulocytes
Authors: LA Ricaurte-C, A Lovera, DA Moreno-Pér, MD Bohórquez, CF Suárez, E Gutiérrez-, L Cuy-Chapar, D Garzón-Osp, MA Patarroyo
International Journal of Molecular Sciences, 2021;22(4):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Targeting the ABC transporter ABCB5 sensitizes glioblastoma to temozolomide-induced apoptosis through a cell-cycle checkpoint regulation mechanism
Authors: CAA Lee, P Banerjee, BJ Wilson, S Wu, Q Guo, G Berg, S Karpova, A Mishra, JW Lian, J Tran, M Emmerich, GF Murphy, MH Frank, NY Frank
J. Biol. Chem., 2020;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Development of an ex vivo xenogeneic bone environment producing human platelet-like cells
Authors: S Fujiyama, N Hori, T Sato, S Enosawa, M Murata, E Kobayashi
PLoS ONE, 2020;15(4):e0230507.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Impact of p53 status on TRAIL-mediated apoptotic and non-apoptotic signaling in cancer cells
Authors: A Willms, H Schittek, S Rahn, J Sosna, U Mert, D Adam, A Trauzold
PLoS ONE, 2019;14(4):e0214847.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Pro-inflammatory monocyte profile in patients with major depressive disorder and suicide behaviour and how ketamine induces anti-inflammatory M2 macrophages by NMDAR and mTOR
Authors: W Nowak, LN Grendas, LM Sanmarco, IG Estecho, ÁR Arena, N Eberhardt, DE Rodante, MP Aoki, FM Daray, EA Carrera Si, AE Errasti
EBioMedicine, 2019;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Increase in bone metabolic markers and circulating osteoblast-lineage cells after orthognathic surgery
Authors: Y Abe, M Chiba, S Yaklai, RS Pechayco, H Suzuki, T Takahashi
Sci Rep, 2019;9(1):20106.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Targeting chemoresistant colorectal cancer via systemic administration of a BMP7 variant
Authors: V Veschi, LR Mangiapane, A Nicotra, S Di Franco, E Scavo, T Apuzzo, DS Sardina, M Fiori, A Benfante, ML Colorito, G Cocorullo, F Giuliante, C Cipolla, G Pistone, MR Bongiorno, A Rizzo, CM Tate, X Wu, S Rowlinson, LF Stancato, M Todaro, R De Maria, G Stassi
Oncogene, 2019;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
TRAIL Induces Nuclear Translocation and Chromatin Localization of TRAIL Death Receptors
Authors: U Mert, A Adawy, E Scharff, P Teichmann, A Willms, V Haselmann, C Colmorgen, J Lemke, S von Karste, J Fritsch, A Trauzold
Cancers (Basel), 2019;11(8):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Induction of MET Receptor Tyrosine Kinase Down-regulation through Antibody-mediated Receptor Clustering
Authors: W Li, A Dick, F Lu, H Zhang, H Sun
Sci Rep, 2019;9(1):1988.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
CXCR7 expression in diffuse large B-cell lymphoma identifies a subgroup of CXCR4+ patients with good prognosis
Authors: MJ Moreno, A Gallardo, S Novelli, A Mozos, M Aragó, MÁ Pavón, MV Céspedes, V Pallarès, A Falgàs, M Alcoceba, O Blanco, M Gonzalez-D, J Sierra, R Mangues, I Casanova
PLoS ONE, 2018;13(6):e0198789.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Regeneration of the lung alveolus by an evolutionarily conserved epithelial progenitor
Authors: WJ Zacharias, DB Frank, JA Zepp, MP Morley, FA Alkhaleel, J Kong, S Zhou, E Cantu, EE Morrisey
Nature, 2018;555(7695):251-255.
Species: Human
Sample Types: Whole Cells
Applications: Control -
Equine Mesenchymal Stromal Cells retain a pericyte-like phenotype
Authors: CL Esteves, TA Sheldrake, L Dawson, T Menghini, E Rink, K Amilon, N Khan, B Peault, X Donadeu
Stem Cells Dev., 2017;0(0):.
Species: Equine
Sample Types: Whole Cells
Applications: Flow Cytometry -
Biological Activity of Masked Endotoxin
Authors: H Schwarz, J Gornicec, T Neuper, MA Parigiani, M Wallner, A Duschl, J Horejs-Hoe
Sci Rep, 2017;7(0):44750.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry Control -
Differential cytokine contributions of perivascular haematopoietic stem cell niches
Authors: N Asada, Y Kunisaki, H Pierce, Z Wang, NF Fernandez, A Birbrair, A Ma'ayan, PS Frenette
Nat. Cell Biol, 2017;19(3):214-223.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Role of IL-18 in atopic asthma is determined by balance of IL-18/IL-18BP/IL-18R
Authors: H Zhang, J Wang, L Wang, H Xie, L Chen, S He
J. Cell. Mol. Med., 2017;0(0):.
Applications: Flow Cytometry Control -
Signaling via PI3K/FOXO1A pathway modulates formation and survival of human embryonic stem cell-derived endothelial cells.
Authors: Merkely B, Gara E, Lendvai Z, Skopal J, Leja T, Zhou W, Kosztin A, Varady G, Mioulane M, Bagyura Z, Nemeth T, Harding S, Foldes G
Stem Cells Dev, 2015;24(7):869-78.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Prerequisites for Functional Interleukin 31 Signaling and Its Feedback Regulation by Suppressor of Cytokine Signaling 3 (SOCS3).
Authors: Maier E, Mittermeir M, Ess S, Neuper T, Schmiedlechner A, Duschl A, Horejs-Hoeck J
J Biol Chem, 2015;290(41):24747-59.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Cancer stem-like sphere cells induced from de-differentiated hepatocellular carcinoma-derived cell lines possess the resistance to anti-cancer drugs.
Authors: Hashimoto, Noriaki, Tsunedomi, Ryouichi, Yoshimura, Kiyoshi, Watanabe, Yusaku, Hazama, Shoichi, Oka, Masaaki
BMC Cancer, 2014;14(0):722.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry Control -
The Spiegelmer NOX-A12, a novel CXCL12 inhibitor, interferes with chronic lymphocytic leukemia cell motility and causes chemosensitization.
Authors: Hoellenriegel J, Zboralski D, Maasch C, Rosin N, Wierda W, Keating M, Kruschinski A, Burger J
Blood, 2014;123(7):1032-9.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
VEGFR2 expressing circulating (progenitor) cell populations in volunteers and cancer patients.
Authors: Vroling L, Yuana Y, Schuurhuis GJ, van Hinsbergh VW, Gundy C, de Haas R, van Cruijsen H, Boven E, Hoekman K, Broxterman HJ
Thromb. Haemost., 2007;98(2):440-50.
Species: Human
Sample Types: Whole Blood
Applications: Control -
Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration.
Authors: Li Y, Fan J, Chen M, Li W, Woodley DT
J. Invest. Dermatol., 2006;126(9):2096-105.
Species: Human
Sample Types: Serum
Applications: Control
FAQs
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Reviews for Mouse IgG1 APC-conjugated Antibody
Average Rating: 4.3 (Based on 3 Reviews)
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Mouse IgG1 APC-conjugated Antibody
By Abby Sukarto
on 02/14/2020
Application: Flow
Sample Tested: Bone Extracts
Species:
Human
Mouse IgG1 APC-conjugated Antibody
By Anonymous
on 09/10/2016
Application: Flow
Sample Tested: ASTROCYTE
Species:
Human
Mouse IgG1 APC-conjugated Antibody
By Anonymous
on 06/15/2016
Application: Flow
Sample Tested: Human embryonic stem cells derived mesoderm (H1)
Species:
Human
1 million of H1 embryonic stem cells were incubated with 10ul of mouse IgG1 APC-conjugated and 10 ul of mouse IgG1 PE-conjugated Antibodies for 45min at 4C in dark. Cells were analyzed user the LSRII analyzer.
