Claudin-11 is a 207 amino acid multipass membrane protein in the claudin family of epithelial tight junction proteins. Claudin-11 is widely expressed and is involved in epithelial remodeling during spermatogenesis and inner ear mechanotransduction. Claudin-11 is up‑regulated in some cancers. Human Claudin-11 shares 94% aa sequence identity with mouse and rat Claudin-11.
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Discontinued Product
MAB4280 has been discontinued.
An alternative/replacement product is available:
MAB4280R. View all Claudin-11 products.
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 398205
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Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human Claudin-11
Met1-Val207
Accession # O75508
Met1-Val207
Accession # O75508
Specificity
Detects human Claudin‑11 in direct ELISAs and Western blots. Stains human Claudin-11 transfectants but not irrelevant transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Claudin-11 Antibody (398205)
Detection of Claudin‑11 in OVCAR‑3 Human Cell Line by Flow Cytometry.
OVCAR-3 human ovarian carcinoma cell line was stained with Mouse Anti-Human Claudin-11 Monoclonal Antibody (Catalog # MAB4280, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).Applications for Human Claudin-11 Antibody (398205)
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: OVCAR‑3 human ovarian carcinoma cell line
Sample: OVCAR‑3 human ovarian carcinoma cell line
Reviewed Applications
Read 1 review rated 4 using MAB4280 in the following applications:
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Claudin-11
Alternate Names
Claudin11, CLDN11, OSP, OTM
Gene Symbol
CLDN11
UniProt
Additional Claudin-11 Products
Product Documents for Human Claudin-11 Antibody (398205)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Claudin-11 Antibody (398205)
For research use only
Related Research Areas
Customer Reviews for Human Claudin-11 Antibody (398205) (1)
4 out of 5
1 Customer Rating
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Application: ImmunocytochemistrySample Tested: pig SC cellsSpecies: PigVerified Customer | Posted 06/07/2017Mosue monoclonal anti-clauidn-11 antibody (398205) labeling pig SC cells with Alexa 594
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
