Human Claudin‑11 Antibody

R&D Systems | Catalog # MAB4280

R&D Systems
Discontinued Product
MAB4280 has been discontinued. An alternative/replacement product is available: MAB4280R. View all Claudin-11 products.

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 398205
View all Claudin-11 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human Claudin-11
Met1-Val207
Accession # O75508

Specificity

Detects human Claudin‑11 in direct ELISAs and Western blots. Stains human Claudin-11 transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Claudin‑11 Antibody

Detection of Claudin‑11 antibody in OVCAR‑3 Human Cell Line antibody by Flow Cytometry.

Detection of Claudin‑11 in OVCAR‑3 Human Cell Line by Flow Cytometry.

OVCAR-3 human ovarian carcinoma cell line was stained with Mouse Anti-Human Claudin-11 Monoclonal Antibody (Catalog # MAB4280, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).

Applications for Human Claudin‑11 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: OVCAR‑3 human ovarian carcinoma cell line

Reviewed Applications

Read 1 review rated 4 using MAB4280 in the following applications:

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Claudin-11

Claudin-11 is a 207 amino acid multipass membrane protein in the claudin family of epithelial tight junction proteins. Claudin-11 is widely expressed and is involved in epithelial remodeling during spermatogenesis and inner ear mechanotransduction. Claudin-11 is up‑regulated in some cancers. Human Claudin-11 shares 94% aa sequence identity with mouse and rat Claudin-11.

Alternate Names

Claudin11, CLDN11, OSP, OTM

Entrez Gene IDs

5010 (Human); 18417 (Mouse); 84588 (Rat)

Gene Symbol

CLDN11

UniProt

O75508

Additional Claudin-11 Products

Product Documents for Human Claudin‑11 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Claudin‑11 Antibody

For research use only

Customer Reviews for Human Claudin‑11 Antibody (1)

4 out of 5
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Customer Images

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  • Human Claudin-11 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: pig SC cells
    Species: Pig
    Verified Customer | Posted 06/07/2017
    Mosue monoclonal anti-clauidn-11 antibody (398205) labeling pig SC cells with Alexa 594
    Human Claudin‑11 Antibody MAB4280

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