Human CLEC10A/CD301 Alexa Fluor® 488-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human CLEC10A/CD301 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human ASGPR1 or recombinant mouse MGL1 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 744812
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CLEC10A/CD301
    Accession # Q8IUN9
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CLEC10A/CD301 in Human Dendritic Cells by Flow Cytometry. Human immature dendritic cells were stained with Mouse Anti-Human CLEC10A/CD301 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB48881G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CLEC10A/CD301

CLEC10A, also known as macrophage galactose/N-acetyl-galactosamine (GalNAc) specific lectin (MGL), CD301, DC-ASGPR, and HML, is a 40 kDa type II transmembrane glycoprotein that belongs to the C-type lectin family (1). Human and rat carry a single gene for CLEC10A/CD301, while mouse has two closely related MGL1 and MGL2 genes. Human CLEC10A/CD301 consists of a 39 amino acid (aa) cytoplasmic region, a 21 aa transmembrane segment and a 256 aa extracellular domain (ECD) with one carbohydrate recognition domain (CRD) and a neck region (2). Within the CRD, human CLEC10A/CD301 shares 64%-70% aa sequence identity with mouse MGL1, mouse MGL2, and rat MGL. Alternate splicing generates multiple isoforms of human CLEC10A/CD301 with 27 aa, 3 aa, and/or 4 aa deletions within the ECD (3, 4). CLEC10A/CD301 is expressed on immature myleloid dendritic cells and alternatively activated (tolerogenic) macrophages and is upregulated by the immunosuppressant dexamethasone (3-7). CLEC10A/CD301 selectively binds and internalizes terminal nonsialylated alpha - or beta -linked GalNAc moieties on O-linked carbohydrates, including the Tn carcinoma antigen (2-4, 8, 9). Similar ligand preference is exhibited by mouse MGL2 but not MGL1 (10). CLEC10A/CD301 expressed on tolerogenic dendritic cells binds carbohydrate determinants on CD45 (RA, RB, and RC but not RO isoforms) expressed by T, NK, and B cells (6). This interaction inhibits effector T cell activation and induces their apoptosis (6). CLEC10A/CD301 also binds the GP envelope glycoprotein on Marburg and Ebola viruses and enhances viral entry and infectivity (11).

  • References:
    1. Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
    2. Suzuki, N. et al. (1996) J. Immunol. 156:128. 
    3. Higashi, N. et al. (2002) J. Biol. Chem. 277:20686. 
    4. Valladeau, J. et al. (2001) J. Immunol. 167:5767.
    5. van Vliet, S.J. et al. (2006) Immunobiology 211:577. 
    6. van Vliet, S.J. et al. (2006) Nat. Immunol. 7:1200.
    7. Raes, G. et al. (2005) J. Leukoc. Biol. 77:321.
    8. van Vliet, S.J. et al. (2005) Int. Immunol. 17:661.
    9. Higashi, N. et al. (2002) Int. Immunol. 14:545.
    10. Tsuiji, M. et al. (2002) J. Biol. Chem. 277:28892.
    11. Takada, A. et al. (2004) J. Virol. 78:2943.
  • Long Name:
    Macrophage Galactose N-Acetyl-Galactosamine Specific Lectin 1
  • Entrez Gene IDs:
    10462 (Human)
  • Alternate Names:
    CD301 antigen; CD301; CD301C-type lectin domain family 10 member A; CLECSF13; CLECSF14; CLECSF14macrophage C-type lectin; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 14 (macrophage-derived); C-type lectin domain family 10, member A; C-type lectin superfamily member 14; HML2; HMLC-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 13 (macrophage-derived); macrophage lectin 2 (calcium dependent); Macrophage lectin 2; MGL
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Alexa Fluor® 488-conjugated Isotype Control

Ctrl IC0041G  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human CLEC10A/CD301 Alexa Fluor® 488-conjugated Antibody?

Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Certificate of Analysis Lookup

The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

Certificate of Analysis Lookup

Research Areas of Interest
R&D Systems Guarantee


Customer Information