Mouse IgG2B Alexa Fluor® 488-conjugated Isotype Control

Catalog # Availability Size / Price Qty
Detection of Mouse IgG Isotype Control by Flow Cytometry
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Product Details
Citations (6)
Supplemental Products

Mouse IgG2B Alexa Fluor® 488-conjugated Isotype Control Summary

Selected for use as mouse IgG2B isotype control for flow cytometry applications.
Monoclonal Mouse IgG2B Clone # 133303
Protein A or G purified from hybridoma culture supernatant
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)


Recommended Concentration
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P(fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 5 μL/106 cells.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Detection of Mouse IgG Control by Flow Cytometry Human peripheral blood mononuclear cell (PBMCs) were stained with Mouse Anti-Human Plexin A1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB65361G, filled histogram) or Mouse IgG Alexa Fluor® 488-conjugated Isotype Control Antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2B Fc
Alternate Names

Product Datasheets

Citations for Mouse IgG2B Alexa Fluor® 488-conjugated Isotype Control

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Single-cell analysis reveals fibroblast clusters linked to immunotherapy resistance in cancer
    Authors: Y Kieffer, HR Hocine, G Gentric, F Pelon, C Bernard, B Bourachot, S Lameiras, L Albergante, C Bonneau, A Guyard, K Tarte, A Zinovyev, S Baulande, G Zalcman, A Vincent-Sa, F Mechta-Gri
    Cancer Discov, 2020;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. A PKA/cdc42 Signaling Axis Restricts Angiogenic Sprouting by Regulating Podosome Rosette Biogenesis and Matrix Remodeling
    Authors: JL MacKeil, P Brzezinska, J Burke-Klei, AW Craig, CJB Nicol, DH Maurice
    Sci Rep, 2019;9(1):2385.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Evaluation of commonly used ectoderm markers in iPSC trilineage differentiation
    Authors: YL Kuang, A Munoz, G Nalula, KE Santostefa, V Sanghez, G Sanchez, N Terada, AN Mattis, M Iacovino, C Iribarren, RM Krauss, MW Medina
    Stem Cell Res, 2019;37(0):101434.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Dual role of DR5 in death and survival signaling leads to TRAIL resistance in cancer cells
    Authors: Y Shlyakhtin, V Pavet, H Gronemeyer
    Cell Death Dis, 2017;8(8):e3025.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Tumour-associated macrophages secrete pleiotrophin to promote PTPRZ1 signalling in glioblastoma stem cells for tumour growth
    Authors: Y Shi, YF Ping, W Zhou, ZC He, C Chen, BS Bian, L Zhang, L Chen, X Lan, XC Zhang, K Zhou, Q Liu, H Long, TW Fu, XN Zhang, MF Cao, Z Huang, X Fang, X Wang, H Feng, XH Yao, SC Yu, YH Cui, X Zhang, JN Rich, S Bao, XW Bian
    Nat Commun, 2017;8(0):15080.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Base-modified UDP-sugars reduce cell surface levels of P-selectin glycoprotein 1 (PSGL-1) on IL-1?-stimulated human monocytes
    Authors: Varsha Kanabar
    Glycobiology, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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