Mouse IgG2B Alexa Fluor® 488-conjugated Isotype Control

(2 citations)   
  • Specificity
    Selected for use as mouse IgG2B isotype control for flow cytometry applications.
  • Source
    Monoclonal Mouse IgG2B Clone # 133303
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    KLH
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Applications
  •  
    Recommended
    Concentration
    Sample
  • Control
    Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P(fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 5 μL/106 cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse IgGControl by Flow Cytometry Human peripheral blood mononuclear cell (PBMCs) were stainedwith Mouse Anti-Human Plexin A1 Alexa Fluor® 488‑conjugatedMonoclonal Antibody (Catalog # FAB65361G,filled histogram) or Mouse IgG Alexa Fluor® 488-conjugated IsotypeControl Antibody (Catalog # IC0041G, open histogram). View our protocol for StainingMembrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G2B Fc
  • Alternate Names:
    IgG2B
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Species
Applications
Sample Type
  1. Tumour-associated macrophages secrete pleiotrophin to promote PTPRZ1 signalling in glioblastoma stem cells for tumour growth
    Authors: Y Shi, YF Ping, W Zhou, ZC He, C Chen, BS Bian, L Zhang, L Chen, X Lan, XC Zhang, K Zhou, Q Liu, H Long, TW Fu, XN Zhang, MF Cao, Z Huang, X Fang, X Wang, H Feng, XH Yao, SC Yu, YH Cui, X Zhang, JN Rich, S Bao, XW Bian
    Nat Commun, 2017;8(0):15080.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Base-modified UDP-sugars reduce cell surface levels of P-selectin glycoprotein 1 (PSGL-1) on IL-1?-stimulated human monocytes
    Authors: Varsha Kanabar
    Glycobiology, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

Ctrl MAB004 32  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2B PE-conjugated Antibody

Ctrl IC0041P 20  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2B APC-conjugated Antibody

Ctrl IC0041A 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2B Fluorescein‑conjugated Control Antibody

Ctrl IC0041F 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2B Isotype Control

Ctrl MAB0042 6
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2B Alexa Fluor® 700-conjugated Isotype Control

Ctrl IC0041N
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2B Alexa Fluor® 647-conjugated Isotype Control

Ctrl IC0041R
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2B PerCP-conjugated Isotype Control

Ctrl IC0041C
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2B Biotinylated Isotype Control

Ctrl IC0041B 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2B Alexa Fluor® 594-conjugated Isotype Control

Ctrl IC0041T
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2B Alexa Fluor® 405-conjugated Isotype Control

Ctrl IC0041V
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2B Alexa Fluor® 750-conjugated Isotype Control

Ctrl IC0041S
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Mouse IgG2B Alexa Fluor® 488-conjugated Isotype Control?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information