Human Coagulation Factor VII Alexa Fluor® 750-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB23381S-100UG
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Human Coagulation Factor VII Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Coagulation Factor VII in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Kallikrein‑1, -2, -3,
-4, -5, -7, -8, -9, -10, -11, -12, -13, -14, -15, -B1, rhHGFA, rhFactor X, rhFactor XI, rhThrombin, rhuPA, or rhPROC is observed.
Source
Monoclonal Mouse IgG2b Clone # 321605
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor VII
Ala39-Pro444
Accession # NP_062562
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
HASMC human aortic smooth muscle cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Coagulation Factor VII

Coagulation Factors VII and VIIa refer to the pro and active forms of the same protease, respectively (1). Factor VII is synthesized in the liver and circulates in the plasma where it binds to tissue factor (TF), an integral membrane protein found in a variety of cell types. Upon binding of TF, Factor VII is rapidly converted into VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis (2). The cleavage and activation of Coagulation Factors VII, IX and X by VIIa:TF is phospholipid-dependent whereas the cleavage of small peptide substrates is not (1). The predominant splicing variant of Factor VII in normal liver corresponds to the 444 amino acid precursor (3, 4). After a signal peptide (residues 1-38), the mature chain can be further processed into the light chain (residues 39-190) and the heavy chain (residues 191-444). The purified rhFactor VII corresponds to the mature chain, which can be processed and activated by treatment with thermolysin and binding with rhTissue Factor (R&D Systems, Catalog # 2339-PA) under the conditions described above.

References
  1. Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 1659. 
  2. Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009.
  3. Hagen, F.S. et al. (1986) Proc. Natl. Acad. Sci. USA 83:2412.
  4. O’Hara, P.J. et al. (1987) Proc. Natl. Acad. Sci. USA 84:5158.
Long Name
Coagulation Factor VII (Serum Prothrombin Conversion Accelerator)
Entrez Gene IDs
2155 (Human); 14068 (Mouse)
Alternate Names
coagulation factor VII (serum prothrombin conversion accelerator); Coagulation Factor VII; EC 3.4.21; EC 3.4.21.21; Eptacog alfa; F7; FVII coagulation protein; proconvertin; Serum prothrombin conversion accelerator; SPCA

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