Detects human Coagulation Factor VII in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Kallikrein‑1, -2, -3, -4, -5, -7, -8, -9, -10, -11, -12, -13, -14, -15, -B1, rhHGFA, rhFactor X, rhFactor XI, rhThrombin, rhuPA, or rhPROC is observed.
Monoclonal Mouse IgG2B Clone # 321605
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor VII Ala39-Pro444 Accession # NP_062562
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
0.25 µg/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Coagulation Factor VII in HASMC Human Cells by Flow Cytometry. HASMC human aortic smooth muscle cells were stained with Mouse Anti-Human Coagulation Factor VII Monoclonal Antibody (Catalog # MAB23381, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Coagulation Factor VII
Coagulation Factors VII and VIIa refer to the pro and active forms of the same protease, respectively (1). Factor VII is synthesized in the liver and circulates in the plasma where it binds to tissue factor (TF), an integral membrane protein found in a variety of cell types. Upon binding of TF, Factor VII is rapidly converted into VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis (2). The cleavage and activation of Coagulation Factors VII, IX and X by VIIa:TF is phospholipid-dependent whereas the cleavage of small peptide substrates is not (1). The predominant splicing variant of Factor VII in normal liver corresponds to the 444 amino acid precursor (3, 4). After a signal peptide (residues 1-38), the mature chain can be further processed into the light chain (residues 39-190) and the heavy chain (residues 191-444). The purified rhFactor VII corresponds to the mature chain, which can be processed and activated by treatment with thermolysin and binding with rhTissue Factor (R&D Systems, Catalog # 2339-PA) under the conditions described above.
Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 1659.
Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009.
Hagen, F.S. et al. (1986) Proc. Natl. Acad. Sci. USA 83:2412.
O’Hara, P.J. et al. (1987) Proc. Natl. Acad. Sci. USA 84:5158.
Coagulation Factor VII (Serum Prothrombin Conversion Accelerator)
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.