Human DARC APC‑conjugated Antibody

R&D Systems | Catalog # FAB4139A

R&D Systems
Discontinued Product
FAB4139A has been discontinued. View all DARC products.

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 358307
View all DARC Primary Antibodies »

Product Specifications

Immunogen

HEK293 human embryonic kidney cell line transfected with human DARC
Met1-Ser336
Accession # Q16570

Specificity

Detects human DARC. Stains human DARC transfected cells but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human DARC APC‑conjugated Antibody

Detection of DARC antibody in Human Red Blood Cells antibody by Flow Cytometry.

Detection of DARC in Human Red Blood Cells by Flow Cytometry.

Human red blood cells were stained with Mouse Anti-Human DARC APC-conjugated Monoclonal Antibody (Catalog # FAB4139A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human DARC APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human red blood cells

Reviewed Applications

Read 1 review rated 4 using FAB4139A in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: DARC

Duffy Antigen Receptor for Chemokines (DARC), also known as Fy glycoprotein and CD234, is a 7TM non-signaling chemokine receptor that is expressed on venular endothelial cells and erythrocytes. DARC binds and mediates the internalization of several inflammatory CC and CXC chemokines. It transports chemokines to the lumenal face of venular endothelium to promote the extravasation of leukocytes to sites of inflammation. DARC polymorphisms are important in blood transfusion compatibility and determine the susceptibility of erythrocytes to Plasmodium vivax infection. Human DARC shares 60% amino acid sequence identity with mouse DARC.

Long Name

Duffy Antigen, Receptor For Chemokines

Alternate Names

ACKR1, CD234, Duffy Antigen, Fy Antigen, GPD, GpFy

Entrez Gene IDs

2532 (Human); 13349 (Mouse)

Gene Symbol

ACKR1

UniProt

Q16570

Additional DARC Products

Product Documents for Human DARC APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human DARC APC‑conjugated Antibody

For research use only

Related Research Areas

Red Blood Cells (RBCs)

Customer Reviews for Human DARC APC‑conjugated Antibody (1)

4 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human DARC APC‑conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card
Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID: 20040767
    Species: Human
    Verified Customer | Posted 01/26/2015

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies