Human DARC APC-conjugated Antibody

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Detection of DARC in Human Red Blood Cells by Flow Cytometry.
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Product Details
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Human DARC APC-conjugated Antibody Summary

Species Reactivity
Detects human DARC. Stains human DARC transfected cells but not irrelevant transfectants.
Monoclonal Mouse IgG2A Clone # 358307
Protein A or G purified from hybridoma culture supernatant
HEK293 human embryonic kidney cell line transfected with human DARC
Accession # Q16570
Supplied in a saline solution containing BSA and Sodium Azide.
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)


Recommended Concentration
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of DARC antibody in Human Red Blood Cells antibody by Flow Cytometry. View Larger

Detection of DARC in Human Red Blood Cells by Flow Cytometry. Human red blood cells were stained with Mouse Anti-Human DARC APC-conjugated Monoclonal Antibody (Catalog # FAB4139A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: DARC

Duffy Antigen Receptor for Chemokines (DARC), also known as Fy glycoprotein and CD234, is a 7TM non-signaling chemokine receptor that is expressed on venular endothelial cells and erythrocytes. DARC binds and mediates the internalization of several inflammatory CC and CXC chemokines. It transports chemokines to the lumenal face of venular endothelium to promote the extravasation of leukocytes to sites of inflammation. DARC polymorphisms are important in blood transfusion compatibility and determine the susceptibility of erythrocytes to Plasmodium vivax infection. Human DARC shares 60% amino acid sequence identity with mouse DARC.

Long Name
Duffy Antigen, Receptor For Chemokines
Entrez Gene IDs
2532 (Human); 13349 (Mouse)
Alternate Names
ACKR1; CCBP1; CD234 antigen; CD234; DARC; Dfy; Duffy Antigen; Duffy antigen/chemokine receptor; Duffy blood group antigen; Duffy blood group, chemokine receptor; Fy Antigen; Fy glycoprotein; FYDuffy blood group; Glycoprotein D; GPD; GPDWBCQ1; GpFy; Plasmodium vivax receptor

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