Human EMP3 Antibody

R&D Systems | Catalog # MAB4505

R&D Systems
Discontinued Product
MAB4505 has been discontinued. View all EMP3 products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Neutralization, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 439949
View all EMP3 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human EMP3
Met1-Glu163
Accession # P54852

Specificity

Detects human EMP. Stains human EMP3 cell transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human EMP3 Antibody

Detection of EMP3 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of EMP3 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells were stained with Mouse Anti-Human EMP3 Monoclonal Antibody (Catalog # MAB4505, filled histogram) or isotype control antibody (MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Detection of EMP3 in PBMC monocytes by Flow Cytometry.

PBMC monocytes were stained with Mouse Anti-Human EMP3 Monoclonal Antibody (Catalog # MAB4505, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004). View our protocol for Staining Intracellular Molecules.

Applications for Human EMP3 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood mononuclear cells fixed with paraformaldehyde and permeabilized with saponin and PBMC monocytes fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004).

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EMP3

Epithelial membrane protein 3 (EMP3) is a multipass transmembrane protein in the peripheral myelin protein 22 family. It is expressed as a 20‑25 kDa molecule depending on the degree of glycosylation. EMP3 interacts with the P2X7 purinergic receptor on monocytes. Its dysregulation is associated with glioma and neuroblastoma. Human EMP3 shares 93% aa sequence identity with mouse and rat EMP3.

Long Name

Epithelial Membrane Protein 3

Alternate Names

HNMP-1, YMP

Entrez Gene IDs

2014 (Human); 13732 (Mouse); 81505 (Rat)

Gene Symbol

EMP3

UniProt

P54852

Additional EMP3 Products

Product Documents for Human EMP3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human EMP3 Antibody

For research use only

Citations for Human EMP3 Antibody

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FAQs for Human EMP3 Antibody

Showing  1 - 1 of 1 FAQ Showing All

  • Q: Why do you recommend intracellular staining with a flow antibody to a membrane expressed protein?

    A: The antibody may recognize an intracellular portion of the protein,or an abundance of the protein may be in an intracellular location. In our hands, the intracellular staining worked better than surface staining.

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