Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

  (12 citations)
(1 Review)
    
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Product Details
Citations (12)
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IgG primary antibodies in immunofluorescent assays. After purification by affinity chromatography, this antibody was adsorbed to eliminate human cross-reactivity and the IgG fraction was further digested with pepsin to generate F(ab')2 fragments which have reduced interactions with cell surface Fc receptors
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse IgG heavy and light chains
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of mouse origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of MouseIgG Primary Antibody by Flow Cytometry Human peripheral blood granulocytes were stained with MouseAnti-Human CD157 Monoclonal Antibody
(Catalog # MAB47361,filled histogram) or isotype control antibody (Catalog # MAB002,open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG SecondaryAntibody (Catalog # F0102B) at10 μL/106 cells.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G
  • Alternate Names:
    IgG; immunoglobulin heavy constant gamma 1 (G1m marker)
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Re-evaluation of IL-10 signaling reveals novel insights on the contribution of the intracellular domain of the IL-10R2 chain
    Authors: RHP Wilbers, DR van Raaij, LB Westerhof, J Bakker, G Smant, A Schots
    PLoS ONE, 2017;12(10):e0186317.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Renal Transplant Patients Biopsied for Cause and Tested for C4d, DSA, and IgG Subclasses and C1q: Which Humoral Markers Improve Diagnosis and Outcomes?
    Authors: JC Cicciarell, NA Lemp, Y Chang, M Koss, K Hacke, N Kasahara, KM Burns, DI Min, R Naraghi, T Shah
    J Immunol Res, 2017;2017(0):1652931.
    Species: Human
    Sample Type: Serum
    Application: Luminex Development
  3. Podocalyxin promotes proliferation and survival in mature B-cell non-Hodgkin lymphoma cells
    Authors: E Tamayo-Orb, L Amo, M Riñón, N Nieto, E Amutio, N Maruri, M Solaun, A Arrieta, S Larrucea
    Oncotarget, 2017;8(59):99722-99739.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. IL-6 trans-signaling is another pathway to upregulate Osteopontin
    Cytokine, 2016;90(0):88-95.
    Species: Human
    Sample Type: Whole Cells
    Application: Secondary
  5. Crosstalk between mitochondrial ROS and depolarization in the potentiation of TRAIL-induced apoptosis in human tumor cells.
    Authors: Suzuki-Karasaki M, Ochiai T, Suzuki-Karasaki Y
    Int J Oncol, 2014;44(2):616-28.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Lipocalin 2, the TNF-like receptor TWEAKR and its ligand TWEAK act downstream of NFAT1 to regulate breast cancer cell invasion.
    Authors: Gaudineau B, Fougere M, Guaddachi F, Lemoine F, de la Grange P, Jauliac S
    J Cell Sci, 2012;125(0):4475-86.
  7. HER3 targeting of adenovirus by fiber modification increases infection of breast cancer cells in vitro, but not following intratumoral injection in mice.
    Authors: MacLeod S, Elgadi M, Bossi G, Sankar U, Pisio A, Agopsowicz K, Sharon D, Graham F, Hitt M
    Cancer Gene Ther, 2012;19(12):888-98.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow control
  8. Thermal- and Oxidative Stress Causes Enhanced Release of NKG2D Ligand-Bearing Immunosuppressive Exosomes in Leukemia/Lymphoma T and B Cells.
    Authors: Hedlund M, Nagaeva O, Kargl D, Baranov V, Mincheva-Nilsson L
    PLoS ONE, 2011;6(2):e16899.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  9. Expression of the ULBP ligands for NKG2D by B-NHL cells plays an important role in determining their susceptibility to rituximab-induced ADCC.
    Authors: Inagaki A, Ishida T, Yano H, Ishii T, Kusumoto S, Ito A, Ri M, Mori F, Ding J, Komatsu H, Iida S, Ueda R
    Int. J. Cancer, 2009;125(1):212-21.
  10. Induction of MHC class I-related chain B (MICB) by 5-aza-2'-deoxycytidine.
    Authors: Tang KF, He CX, Zeng GL, Wu J, Song GB, Shi YS, Zhang WG, Huang AL, Steinle A, Ren H
    Biochem. Biophys. Res. Commun., 2008;370(4):578-83.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  11. Histone deacetylase inhibitors suppress natural killer cell cytolytic activity.
    Authors: Ogbomo H, Michaelis M, Kreuter J, Doerr HW, Cinatl J
    FEBS Lett., 2007;581(7):1317-22.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  12. Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
    Authors: Ebert EC, Mehta V
    Cell. Immunol., 2006;241(1):7-13.
    Species: Human
    Sample Type: Whole Cells
    Application: Secondary
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody F0102B Array F0102B
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Excellent
 Flow Human Anonymous 03/24/2017
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Flow Cytometry Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody F0102B
Flow Cytometry: Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody [F0102B].

Summary

Application Flow Cytometry
Sample Tested Breast cancer cells
Species Human

Other Experimental Details

Other Experimental Details 106 MA-11 cells were incubated with Mouse anti-Human CD44 antibody (red) or Isotype Control IgG (black), followed by staining with 10 uL of PE-conjugated Goat Anti-Mouse secondary antibody (#F0102B).

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