Human FoxP4 Alexa Fluor® 350-conjugated Antibody

Catalog #: AF6854U Datasheet / COA / SDS

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AF6854U-100UG

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Human FoxP4 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human FoxP4 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) FoxP1, rhFoxP2, rhFoxP3, and rhCTBP1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human FoxP4
Pro549-Ser680
Accession # Q8IVH2

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: FoxP4

FoxP4 (Forkhead box protein P4; also FKHLA) is a 70-80 kDa member of the winged helix transcription factor gene family, FoxP subfamily of proteins. It is a transcriptional repressor that is strongly expressed in embryonic gut and lung epithelium, and in neurons both pre-and postnatally. Postnatally, it is found in cerebellar Purkinje cells. During development, it is found in hippocampal hilus, proliferating cells of the ventricular zone, and neurons of the basolateral nucleus of the amygdala. Human FoxP4 is 680 amino acids (aa) in length. It contains a large poly-Gln region (aa 65-219), a C2H2 Zn-finger domain (aa 307-332), a Leu-zipper dimerization motift (aa 349-370) and a forkhead DNA binding domain (aa 467-559). Phosphorylation occurs at Ser360. FoxP4 forms homodimers, and heterodimers with FoxP1 and FoxP2; it does not bind CTBP1. One potential splice form shows a deletion of aa 384-395. Over aa 549-680, human and mouse FoxP4 share 89% aa identity.

Long Name

Forkhead Box P4

Entrez Gene IDs

116113 (Human); 74123 (Mouse); 363185 (Rat)

Alternate Names

FKHLA; FLJ40908; FLJ44184; fork head-related protein like A; Fork head-related protein-like A; forkhead box P4; forkhead box protein P4; FoxP4; hFKHLA; winged-helix repressor FOXP4