Human Integrin  beta 6 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB4155A
Detection of Integrin  beta 6 in HT‑29 Human Cell Line by Flow Cytometry.
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Human Integrin  beta 6 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin beta 6 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 437211
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Integrin alpha V beta 6
Phe31-Val992 (Integrin alpha V) and Gly22-Asn707 (Integrin beta 6)
Accession # P18564 (Integrin beta 6)
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Integrin  beta 6 antibody in HT-29 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Integrin beta 6 in HT‑29 Human Cell Line by Flow Cytometry. HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human Integrin beta 6 APC-conjugated Monoclonal Antibody (Catalog # FAB4155A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Integrin beta 6

Integrin beta 6 (ITG beta 6) is a 95 kDa member of the Integrin beta family. It forms noncovalent heterodimers with Integrin alpha V and appears on epithelia following injury or inflammation. It activates TGF-beta and assists keratinocyte migration. Human Integrin beta 6 is a type I transmembrane glycoprotein that is 767 amino acids (aa) in length. It contains a 688 aa extracellular domain (ECD) (aa 22-709) that incorporates a 241 aa VWF-A domain. Over aa 22-707, human integrin beta 6 ECD shares 90% and 93% aa sequence identity with mouse and pig integrin beta 6 ECD, respectively.

Entrez Gene IDs
3694 (Human); 16420 (Mouse); 311061 (Rat); 102122296 (Cynomolgus Monkey)
Alternate Names
Integrin beta 6; integrin beta-6; integrin, beta 6; ITGB6

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