Human LSECtin/CLEC4G APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB2947A
Detection of LSECtin/CLEC4G in Human Mature Dendritic Cells by Flow Cytometry.
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Citations (4)
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Human LSECtin/CLEC4G APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human LSECtin/CLEC4G in flow cytometry.
Source
Monoclonal Mouse IgG2A Clone # 845404
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LSECtin/CLEC4G
Ser54-Cys293
Accession # Q6UXB4
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of LSECtin/CLEC4G antibody in Human Mature Dendritic Cells antibody by Flow Cytometry. View Larger

Detection of LSECtin/CLEC4G in Human Mature Dendritic Cells by Flow Cytometry. Human mature dendritic cells were stained with Mouse Anti-Human LSECtin/CLEC4G APC-conjugated Monoclonal Antibody (Catalog # FAB2947A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: LSECtin/CLEC4G

LSECtin (liver and lymph node sinusoidal endothelial cell C-type lectin), also known as C-type lectin superfamily 4, member G (CLEC4G), is a member of subgroup II of the C-type (Ca2+-dependent) lectin superfamily (1). The protein was named LSECtin because its initial expression was described to be restricted to liver and lymph node sinusoidal endothelial cells (1). Since then, however, LSECtin has also been detected in peripheral blood and thymic dendritic cells isolated ex vivo, and in monocyte-derived macrophages and dendritic cells at the RNA and protein level (2). Human LSECtin is an approximately 40 kDa, single-pass, type II transmembrane glycoprotein that is 293 amino acids (aa) in length. It contains a short N-terminal cytoplasmic tail (aa 1‑31) and a 21 aa transmembrane region. Its extracellular region consists of two N-linked glycosylation sites (aa 73 and 159), a coil‑coil neck domain (aa 96‑136), a C‑type lectin-like domain (CTLD) of the type found in human DC‑SIGN and DC‑SIGN receptor (aa 165‑289), and a C-terminal Ca2+-dependent carbohydrate‑recognition domain (C-type CRD) (1). Human LSECtin shares 64% aa sequence identity with mouse LSECtin. LSECtin binds to mannose, GlcNAc, and fucose in a Ca2+-dependent manner (1‑3). In addition, LSECtin has the ability to bind to surface glycoproteins of enveloped viruses (3, 4). In particular, interaction of LSECtin with the surface glycoproteins of severe acute respiratory syndrome (SARS) coronavirus and Ebola virus has been described, and LSECtin‑mediated infection of cells by Ebola virus has been demonstrated (3, 4).

References
  1. Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
  2. Dominguez-Soto, A. et al. (2007) Blood 109:5337.
  3. Powlesland, A. et al. (2008) J. Biol. Chem. 283:593.
  4. Gramberg, T. et al. (2005) Virology 340:224.
Long Name
Liver And Lymph Node Sinusoidal Endothelial Cell C-type Lectin
Entrez Gene IDs
339390 (Human); 75863 (Mouse)
Alternate Names
CLEC4G; C-type lectin domain family 4 member G; C-type lectin domain family 4, member G; C-type lectin superfamily 4, member G; DTTR431; liver and lymph node sinusoidal endothelial cell C-type lectin; LP2698; LSECtin; Q6UXB4; UNQ431

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Citations for Human LSECtin/CLEC4G APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Platelets contribute to disease severity in COVID-19
    Authors: Barrett TJ, Bilaloglu S, Cornwell M et al.
    Journal of thrombosis and haemostasis : JTH
  2. Aryl hydrocarbon receptor activity downstream of IL-10 signaling is required to promote regulatory functions in human dendritic cells
    Authors: D Avancini, A Testori, L Fresolone, G Andolfi, M Vuono, V Martinelli, FR Santoni de, S Gregori
    Cell Reports, 2023-03-03;42(3):112193.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  3. Identification of HIV transmitting CD11c+ human epidermal dendritic cells
    Authors: KM Bertram, RA Botting, H Baharlou, JW Rhodes, H Rana, JD Graham, E Patrick, J Fletcher, TM Plasto, NR Truong, C Royle, CM Doyle, O Tong, N Nasr, L Barnouti, MP Kohout, AJ Brooks, MP Wines, P Haertsch, J Lim, MP Gosselink, G Ctercteko, JD Estes, MJ Churchill, PU Cameron, E Hunter, MA Haniffa, AL Cunningham, AN Harman
    Nat Commun, 2019-06-21;10(1):2759.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Phenotypic and functional consequences of different isolation protocols on skin mononuclear phagocytes
    Authors: RA Botting, KM Bertram, H Baharlou, KJ Sandgren, J Fletcher, JW Rhodes, H Rana, TM Plasto, XM Wang, JJ Lim, L Barnouti, MP Kohout, T Papadopoul, S Merten, N Olbourne, AL Cunningham, M Haniffa, AN Harman
    J. Leukoc. Biol, 2017-03-07;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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