Human LSECtin/CLEC4G APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human LSECtin/CLEC4G in flow cytometry.
  • Source
    Monoclonal Mouse IgG2A Clone # 845404
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human LSECtin/CLEC4G
    Accession # Q6UXB4
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of LSECtin/CLEC4G in Human Mature Dendritic Cells by Flow Cytometry. Human mature dendritic cells were stained with Mouse Anti-Human LSECtin/CLEC4G APC‑conjugated Monoclonal Antibody (Catalog # FAB2947A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: LSECtin/CLEC4G

LSECtin (liver and lymph node sinusoidal endothelial cell C-type lectin), also known as C-type lectin superfamily 4, member G (CLEC4G), is a member of subgroup II of the C-type (Ca2+-dependent) lectin superfamily (1). The protein was named LSECtin because its initial expression was described to be restricted to liver and lymph node sinusoidal endothelial cells (1). Since then, however, LSECtin has also been detected in peripheral blood and thymic dendritic cells isolated ex vivo, and in monocyte-derived macrophages and dendritic cells at the RNA and protein level (2). Human LSECtin is an approximately 40 kDa, single-pass, type II transmembrane glycoprotein that is 293 amino acids (aa) in length. It contains a short N-terminal cytoplasmic tail (aa 1‑31) and a 21 aa transmembrane region. Its extracellular region consists of two N-linked glycosylation sites (aa 73 and 159), a coil‑coil neck domain (aa 96‑136), a C‑type lectin-like domain (CTLD) of the type found in human DC‑SIGN and DC‑SIGN receptor (aa 165‑289), and a C-terminal Ca2+-dependent carbohydrate‑recognition domain (C-type CRD) (1). Human LSECtin shares 64% aa sequence identity with mouse LSECtin. LSECtin binds to mannose, GlcNAc, and fucose in a Ca2+-dependent manner (1‑3). In addition, LSECtin has the ability to bind to surface glycoproteins of enveloped viruses (3, 4). In particular, interaction of LSECtin with the surface glycoproteins of severe acute respiratory syndrome (SARS) coronavirus and Ebola virus has been described, and LSECtin‑mediated infection of cells by Ebola virus has been demonstrated (3, 4).

  • References:
    1. Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
    2. Dominguez-Soto, A. et al. (2007) Blood 109:5337.
    3. Powlesland, A. et al. (2008) J. Biol. Chem. 283:593.
    4. Gramberg, T. et al. (2005) Virology 340:224.
  • Long Name:
    Liver And Lymph Node Sinusoidal Endothelial Cell C-type Lectin
  • Entrez Gene IDs:
    339390 (Human); 75863 (Mouse)
  • Alternate Names:
    CLEC4G; C-type lectin domain family 4 member G; C-type lectin domain family 4, member G; C-type lectin superfamily 4, member G; DTTR431; liver and lymph node sinusoidal endothelial cell C-type lectin; LP2698; LSECtin; Q6UXB4; UNQ431
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A APC-conjugated Antibody

Ctrl IC003A 1  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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