Human/Mouse SSEA-3 Alexa Fluor® 488-conjugated Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Recognizes a carbohydrate epitope of SSEA-3 (4, 5).
  • Source
    Monoclonal Rat IgM Clone # MC-631
  • Purification
    IgM-specific Affinity-purified from hybridoma culture supernatant
  • Immunogen
    Four to eight cell stage mouse embryos
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of SSEA‑3 in BG01V Human Embryonic Stem Cells by Flow Cytometry. BG01V human embryonic stem cells was stained with Rat Anti-Human/Mouse SSEA‑3 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1434G, filled histogram) or isotype control antibody (open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of SSEA‑3 in NTera‑2 Human Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line was stained with Rat Anti-Human/Mouse SSEA‑3 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1434G, filled histogram) or isotype control antibody (open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: SSEA-3

SSEA-3, also known as glycolipid GB5, is expressed on the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), and human embryonic stem cells (ES) (1). Expression of SSEA-3 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-3 expression (2, 3).

  • References:
    1. Zhou, D. et al. (2000) J. Biol. Chem. 275:22631.
    2. Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53.
    3. Draper, J.S. et al. (2002) J. Anat. 200:249.
    4. Shevinsky, L.H. et al. (1982) Cell 30:697.
    5. Kannagi, R. et al. (1983) EMBO J. 2:2355.
  • Long Name:
    Stage-specific Embryonic Antigen-3
  • Alternate Names:
    SSEA3; SSEA-3; Stage specific embryonic antigen 3; Stage-specific embryonic antigen 3
Related Research Areas
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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