Human/Mouse SSEA-3 Antibody

Catalog # Availability Size / Price Qty
MAB1434
MAB1434-SP
Human/Mouse SSEA-3 Antibody in Data
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Product Details
Citations (16)
FAQs
Supplemental Products
Reviews (5)

Human/Mouse SSEA-3 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Recognizes a carbohydrate epitope of SSEA-3 (4, 5).
Source
Monoclonal Rat IgM Clone # MC-631
Purification
IgM-specific Affinity-purified from hybridoma culture supernatant
Immunogen
Four to eight cell stage mouse embryos
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
D3 mouse embryonic stem cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry SSEA-3 antibody in BG01V Human Stem Cells by Immunocytochemistry (ICC). View Larger

SSEA‑3 in BG01V Human Stem Cells. SSEA-3 was detected in immersion fixed non-permeabilized BG01V human embryonic stem cells using Human/Mouse SSEA-3 Monoclonal Antibody (Catalog # MAB1434) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SSEA-3

SSEA-3, also known as glycolipid GB5, is expressed on the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), and human embryonic stem cells (ES) (1). Expression of SSEA-3 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-3 expression (2, 3).

References
  1. Zhou, D. et al. (2000) J. Biol. Chem. 275:22631.
  2. Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53.
  3. Draper, J.S. et al. (2002) J. Anat. 200:249.
  4. Shevinsky, L.H. et al. (1982) Cell 30:697.
  5. Kannagi, R. et al. (1983) EMBO J. 2:2355.
Long Name
Stage-specific Embryonic Antigen-3
Alternate Names
SSEA3; SSEA-3; Stage specific embryonic antigen 3; Stage-specific embryonic antigen 3

Product Datasheets

Citations for Human/Mouse SSEA-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Progesterone Receptor Membrane Component 1 suppresses the p53 and Wnt/?-catenin pathways to promote human pluripotent stem cell self-renewal
    Authors: JY Kim, SY Kim, HS Choi, MK Kim, HM Lee, YJ Jang, CJ Ryu
    Sci Rep, 2018;8(1):3048.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Effects of the Extracts from Fruit and Stem of Camellia japonica on Induced Pluripotency and Wound Healing
    Authors: H Jeon, JY Kim, JK Choi, E Han, CL Song, J Lee, YS Cho
    J Clin Med, 2018;7(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. High expression FUT1 and B3GALT5 is an independent predictor of postoperative recurrence and survival in hepatocellular carcinoma
    Authors: HH Kuo, RJ Lin, JT Hung, CB Hsieh, TH Hung, FY Lo, MY Ho, CT Yeh, YL Huang, J Yu, AL Yu
    Sci Rep, 2017;7(1):10750.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Induction of Pluripotent Stem Cells from a Manifesting Carrier of Duchenne Muscular Dystrophy and Characterization of Their X-Inactivation Status
    Authors: Y Miyagoe-Su, T Nishiyama, M Nakamura, A Narita, F Takemura, S Masuda, N Minami, K Murayama, H Komaki, YI Goto, S Takeda
    Stem Cells Int, 2017;2017(0):7906843.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience
    Authors: PA De Sousa, R Steeg, E Wachter, K Bruce, J King, M Hoeve, S Khadun, G McConnachi, J Holder, A Kurtz, S Seltmann, J Dewender, S Reimann, G Stacey, O O'Shea, C Chapman, L Healy, H Zimmermann, B Bolton, T Rawat, I Atkin, A Veiga, B Kuebler, BM Serano, T Saric, J Hescheler, O Brüstle, M Peitz, C Thiele, N Geijsen, B Holst, C Clausen, M Lako, L Armstrong, SK Gupta, AJ Kvist, R Hicks, A Jonebring, G Brolén, A Ebneth, A Cabrera-So, P Foerch, M Geraerts, TC Stummann, S Harmon, C George, I Streeter, L Clarke, H Parkinson, PW Harrison, A Faulconbri, L Cherubin, T Burdett, C Trigueros, MJ Patel, C Lucas, B Hardy, R Predan, J Dokler, M Brajnik, O Keminer, O Pless, P Gribbon, C Claussen, A Ringwald, B Kreisel, A Courtney, TE Allsopp
    Stem Cell Res, 2017;20(0):105-114.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. A Cytotoxic Antibody Recognizing Lacto-N-fucopentaose I (LNFP I) on Human Induced Pluripotent Stem (hiPS) Cells.
    Authors: Matsumoto S, Nakao H, Kawabe K, Nonaka M, Toyoda H, Takishima Y, Kawabata K, Yamaguchi T, Furue M, Taki T, Okumura T, Yamazaki Y, Nakaya S, Kawasaki N, Kawasaki T
    J Biol Chem, 2016;290(33):20071-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  7. Microfabric Vessels for Embryoid Body Formation and Rapid Differentiation of Pluripotent Stem Cells
    Sci Rep, 2016;6(0):31063.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  8. B-cell receptor-associated protein 31 regulates human embryonic stem cell adhesion, stemness, and survival via control of epithelial cell adhesion molecule.
    Authors: Kim W, Seo Choi H, Min Lee H, Jang Y, Ryu C
    Stem Cells, 2014;32(10):2626-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells.
    BMC Vet Res, 2012;8(0):150.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Histone deacetylase inhibition elicits an evolutionarily conserved self-renewal program in embryonic stem cells.
    Authors: Ware CB, Wang L, Mecham BH, Shen L, Nelson AM, Bar M, Lamba DA, Dauphin DS, Buckingham B, Askari B, Lim R, Tewari M, Gartler SM, Issa JP, Pavlidis P, Duan Z, Blau CA
    Cell Stem Cell, 2009;4(4):359-69.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Generation of pluripotent stem cells from adult human testis.
    Authors: Conrad S, Renninger M, Hennenlotter J, Wiesner T, Just L, Bonin M, Aicher W, Buhring HJ, Mattheus U, Mack A, Wagner HJ, Minger S, Matzkies M, Reppel M, Hescheler J, Sievert KD, Stenzl A, Skutella T
    Nature, 2008;456(7220):344-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Regulation of self-renewal and pluripotency by Sox2 in human embryonic stem cells.
    Authors: Fong H, Hohenstein KA, Donovan PJ
    Stem Cells, 2008;26(8):1931-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  13. Secreted proteoglycans directly mediate human embryonic stem cell-basic fibroblast growth factor 2 interactions critical for proliferation.
    Authors: Levenstein ME, Berggren WT, Lee JE, Conard KR, Llanas RA, Wagner RJ, Smith LM, Thomson JA
    Stem Cells, 2008;26(12):3099-107.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  14. Nucleofection mediates high-efficiency stable gene knockdown and transgene expression in human embryonic stem cells.
    Authors: Hohenstein KA, Pyle AD, Chern JY, Lock LF, Donovan PJ
    Stem Cells, 2008;26(6):1436-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  15. Equine umbilical cord blood contains a population of stem cells that express Oct4 and differentiate into mesodermal and endodermal cell types.
    Authors: Reed SA, Johnson SE
    J. Cell. Physiol., 2008;215(2):329-36.
    Species: Equine
    Sample Types: Whole Cells
    Applications: ICC
  16. Multipotent mesenchymal stem cells from human placenta: critical parameters for isolation and maintenance of stemness after isolation.
    Authors: Semenov O, Koestenbauer S, Riegel M, Zech N, Zimmermann R, Zisch A, Malek A
    Am J Obstet Gynecol, 0;202(2):193.e1-193.e1.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human/Mouse SSEA-3 Antibody

Average Rating: 4 (Based on 5 Reviews)

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Human/Mouse SSEA-3 Antibody
By Anonymous on 12/11/2020
Application: ICC/IF Sample Tested: iPS2 human induced pluripotent stem cells Species: Human

I used it to check for SSEA-3 in my iPSCs- beautiful staining


Human/Mouse SSEA-3 Antibody
By Anonymous on 06/10/2020
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human/Mouse SSEA-3 Antibody
By Anonymous on 11/20/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Human/Mouse SSEA-3 Antibody
By Anonymous on 11/11/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Human

Human/Mouse SSEA-3 Antibody
By Anonymous on 11/07/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human