Human/Mouse SSEA-3 Antibody

(12 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Recognizes a carbohydrate epitope of SSEA-3 (4, 5).
  • Source
    Monoclonal Rat IgM Clone # MC-631
  • Purification
    IgM-specific Affinity-purified from hybridoma culture supernatant
  • Immunogen
    Four to eight cell stage mouse embryos
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    D3 mouse embryonic stem cell line
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
SSEA‑3 in BG01V Human Stem Cells. SSEA‑3 was detected in immersion fixed non-permeabilized BG01V human embryonic stem cells using Human/Mouse SSEA‑3 Monoclonal Antibody (Catalog # MAB1434) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SSEA-3

SSEA-3, also known as glycolipid GB5, is expressed on the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), and human embryonic stem cells (ES) (1). Expression of SSEA-3 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-3 expression (2, 3).

  • References:
    1. Zhou, D. et al. (2000) J. Biol. Chem. 275:22631.
    2. Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53.
    3. Draper, J.S. et al. (2002) J. Anat. 200:249.
    4. Shevinsky, L.H. et al. (1982) Cell 30:697.
    5. Kannagi, R. et al. (1983) EMBO J. 2:2355.
  • Long Name:
    Stage-specific Embryonic Antigen-3
  • Alternate Names:
    SSEA3; SSEA-3; Stage specific embryonic antigen 3; Stage-specific embryonic antigen 3
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience
    Authors: PA De Sousa, R Steeg, E Wachter, K Bruce, J King, M Hoeve, S Khadun, G McConnachi, J Holder, A Kurtz, S Seltmann, J Dewender, S Reimann, G Stacey, O O'Shea, C Chapman, L Healy, H Zimmermann, B Bolton, T Rawat, I Atkin, A Veiga, B Kuebler, BM Serano, T Saric, J Hescheler, O Brüstle, M Peitz, C Thiele, N Geijsen, B Holst, C Clausen, M Lako, L Armstrong, SK Gupta, AJ Kvist, R Hicks, A Jonebring, G Brolén, A Ebneth, A Cabrera-So, P Foerch, M Geraerts, TC Stummann, S Harmon, C George, I Streeter, L Clarke, H Parkinson, PW Harrison, A Faulconbri, L Cherubin, T Burdett, C Trigueros, MJ Patel, C Lucas, B Hardy, R Predan, J Dokler, M Brajnik, O Keminer, O Pless, P Gribbon, C Claussen, A Ringwald, B Kreisel, A Courtney, TE Allsopp
    Stem Cell Res, 2017;20(0):105-114.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Induction of Pluripotent Stem Cells from a Manifesting Carrier of Duchenne Muscular Dystrophy and Characterization of Their X-Inactivation Status
    Authors: Y Miyagoe-Su, T Nishiyama, M Nakamura, A Narita, F Takemura, S Masuda, N Minami, K Murayama, H Komaki, YI Goto, S Takeda
    Stem Cells Int, 2017;2017(0):7906843.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  3. A Cytotoxic Antibody Recognizing Lacto-N-fucopentaose I (LNFP I) on Human Induced Pluripotent Stem (hiPS) Cells.
    Authors: Matsumoto S, Nakao H, Kawabe K, Nonaka M, Toyoda H, Takishima Y, Kawabata K, Yamaguchi T, Furue M, Taki T, Okumura T, Yamazaki Y, Nakaya S, Kawasaki N, Kawasaki T
    J Biol Chem, 2016;290(33):20071-85.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC - PFA fixed
  4. Microfabric Vessels for Embryoid Body Formation and Rapid Differentiation of Pluripotent Stem Cells
    Sci Rep, 2016;6(0):31063.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC-Fluorescent
  5. B-cell receptor-associated protein 31 regulates human embryonic stem cell adhesion, stemness, and survival via control of epithelial cell adhesion molecule.
    Authors: Kim W, Seo Choi H, Min Lee H, Jang Y, Ryu C
    Stem Cells, 2014;32(10):2626-41.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells.
    BMC Vet Res, 2012;8(0):150.
    Species: Canine
    Sample Type: Whole Cells
    Application: Flow
  7. Histone deacetylase inhibition elicits an evolutionarily conserved self-renewal program in embryonic stem cells.
    Authors: Ware CB, Wang L, Mecham BH, Shen L, Nelson AM, Bar M, Lamba DA, Dauphin DS, Buckingham B, Askari B, Lim R, Tewari M, Gartler SM, Issa JP, Pavlidis P, Duan Z, Blau CA
    Cell Stem Cell, 2009;4(4):359-69.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  8. Secreted proteoglycans directly mediate human embryonic stem cell-basic fibroblast growth factor 2 interactions critical for proliferation.
    Authors: Levenstein ME, Berggren WT, Lee JE, Conard KR, Llanas RA, Wagner RJ, Smith LM, Thomson JA
    Stem Cells, 2008;26(12):3099-107.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  9. Regulation of self-renewal and pluripotency by Sox2 in human embryonic stem cells.
    Authors: Fong H, Hohenstein KA, Donovan PJ
    Stem Cells, 2008;26(8):1931-8.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  10. Generation of pluripotent stem cells from adult human testis.
    Authors: Conrad S, Renninger M, Hennenlotter J, Wiesner T, Just L, Bonin M, Aicher W, Buhring HJ, Mattheus U, Mack A, Wagner HJ, Minger S, Matzkies M, Reppel M, Hescheler J, Sievert KD, Stenzl A, Skutella T
    Nature, 2008;456(7220):344-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  11. Nucleofection mediates high-efficiency stable gene knockdown and transgene expression in human embryonic stem cells.
    Authors: Hohenstein KA, Pyle AD, Chern JY, Lock LF, Donovan PJ
    Stem Cells, 2008;26(6):1436-43.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  12. Equine umbilical cord blood contains a population of stem cells that express Oct4 and differentiate into mesodermal and endodermal cell types.
    Authors: Reed SA, Johnson SE
    J. Cell. Physiol., 2008;215(2):329-36.
    Species: Equine
    Sample Type: Whole Cells
    Application: ICC
Expand to show all 12 Citations
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