Human NK1R APC-conjugated Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human NK1R in ELISAs.
  • Source
    Monoclonal Mouse IgG3 Clone # 694501
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    HEK293 human embryonic kidney cell line transfected with human NK1R
    Accession # P25103
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of NK1R in THP‑1 Human Cell Line by Flow Cytometry. THP‑1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human NK1R APC‑conjugated Monoclonal Antibody (Catalog # FAB66871A, filled histogram) or isotype control antibody (Catalog # IC007A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: NK1R
NK1R (Neurokinin-1 Receptor), gene name TACR1 (Tachykinin Receptor-1), is an ~80 kDa 7-transmembrane glycoprotein receptor for the proinflammatory tachykinin neuropeptide, Substance P. NK1R is constitutively or inducibly expressed on a wide variety of cells, including monocytes, macrophages, microglia, lymphocytes, neutrophils, mast cells, and neurons. The 407 amino acid (aa) human NK1R contains 89 extracellular aa over multiple segments which collectively share 92% aa identity with corresponding regions of mouse and rat NK1R. An ~50 kDa short isoform that ends at aa 311 lacks almost all of the C-terminal cytoplasmic signaling region. It is the only form expressed in human monocytes and undifferentiated THP-1 cells, and its function appears to differ from the long isoform.
  • Long Name:
    Neurokinin-1 Receptor
  • Entrez Gene IDs:
    6869 (Human); 21336 (Mouse); 24807 (Rat)
  • Alternate Names:
    NK1R; NK-1R; NK1Rneurokinin 1 receptor; NKIR; SPR; SPRsubstance-P receptor; TAC1R; TAC1Rtachykinin 1 receptor (substance P receptor, neurokinin 1 receptor); tachykinin receptor 1 (substance P receptor; neurokinin-1 receptor); tachykinin receptor 1NK-1 receptor; TACR1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. SP and IL-33 together markedly enhance TNF synthesis and secretion from human mast cells mediated by the interaction of their receptors
    Authors: A Taracanova, M Alevizos, A Karagkouni, Z Weng, E Norwitz, P Conti, SE Leeman, TC Theoharide
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG3 APC-conjugated Antibody

Ctrl IC007A 1  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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