Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # 694501
View all NK1R Primary Antibodies »

Product Specifications

Immunogen

HEK293 human embryonic kidney cell line transfected with human NK1R
Accession # P25103

Specificity

Detects human NK1R in ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Scientific Data Images for Human NK1R Antibody

NK1R antibody in THP-1 Human Cell Line by Immunocytochemistry (ICC).

NK1R in THP‑1 Human Cell Line.

NK1R was detected in immersion fixed THP-1 human acute monocytic leukemia cell line using Mouse Anti-Human NK1R Monoclonal Antibody (Catalog # MAB66871) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human NK1R Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed THP‑1 human acute monocytic leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NK1R

NK1R (Neurokinin-1 receptor), gene name TACR1 (tachykinin receptor-1), is an ~80 kDa 7-transmembrane glycoprotein receptor for the proinflammatory tachykinin neuropeptide, substance P. NK1R is constitutively or inducibly expressed on a wide variety of cells, including monocytes, macrophages, microglia, lymphocytes, neutrophils, mast cells, and neurons. The 407 amino acid (aa) human NK1R contains 89 extracellular aa over multiple segments which collectively share 92% aa identity with corresponding regions of mouse and rat NK1R. A ~50 kDa short isoform that ends at aa 311 lacks almost all of the C-terminal cytoplasmic signaling region. It is the only form expressed in human monocytes and undifferentiated THP-1 cells, and its function appears to differ from the long isoform.

Long Name

Neurokinin-1 Receptor

Alternate Names

SPR, TAC1R, TACR1

Entrez Gene IDs

6869 (Human); 21336 (Mouse); 24807 (Rat)

Gene Symbol

TACR1

UniProt

P25103

Additional NK1R Products

Product Documents for Human NK1R Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human NK1R Antibody

For research use only

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