Human NRAMP1/SLC11A1 APC-conjugated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects HEK293 human embryonic kidney cell line transfected with human NRAMP1/SLC11A1 by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.
  • Source
    Monoclonal Mouse IgG2B Clone # 910825
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human NRAMP1/SLC11A1
    Met1-Gly550
    Accession # NP_000569
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of NRAMP1/SLC11A1 in HEK293 Human Cell Line Transfected with Human NRAMP1/SLC11A1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human NRAMP1/SLC11A1 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human NRAMP1/SLC11A1 APC‑conjugated Monoclonal Antibody (Catalog # FAB8400A). Quadrant markers were set based on control antibody staining (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: NRAMP1/SLC11A1
NRAMP1/SLC11A1 (Natural Resistance-Associated Macrophage Protein 1/Solute Carrier family 11, member 1) is a member of the solute carrier family of multi-pass membrane proteins. It functions as a proton-coupled divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Mutations in SLC11A1 have been associated with susceptibility to infectious diseases such as tuberculosis and leprosy, and inflammatory diseases such as rheumatoid arthritis and Crohn’s disease. Alternatively spliced variants that encode different protein isoforms have been described.
  • Long Name:
    Natural Resistance-associated Macrophage Protein 1
  • Entrez Gene IDs:
    6556 (Human); 18173 (Mouse); 316519 (Rat)
  • Alternate Names:
    natural resistance-associated macrophage protein 1; LSH; member 1; NRAMP 1; NRAMP1; NRAMP1solute carrier family 11 (sodium/phosphate symporters), member 1; NRAMPnatural resistance-associated macrophage protein 1; SLC11A1; solute carrier family 11 (proton-coupled divalent metal ion transporters)
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B APC-conjugated Antibody

Ctrl IC0041A 4  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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