Human S1P2/EDG-5 APC-conjugated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human S1P2/EDG-5. Stains human S1P2/EDG-5 transfectants but not irrelevant transfectants.
  • Source
    Monoclonal Mouse IgG1 Clone # 368510
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human S1P2/EDG-5
    Met1-Val353
    Accession # AAC98919
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of S1P2/EDG-5 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human S1P2/EDG-5 APC‑conjugated Monoclonal Antibody (Catalog # FAB3649A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of S1P2/EDG-5 in HEK293 Human Cell Line Transfected with Human S1P2/EDG-5 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human S1P2/EDG-5 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human S1P2/EDG-5 APC‑conjugated Monoclonal Antibody (Catalog # FAB3649A). Quadrant markers were set based on control antibody staining (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: S1P2/EDG-5
S1P2 (sphingosine 1-phosphate receptor-2), also known as EDG-5 (endothelial differentiation, G-protein coupled receptor-5), is a 353 amino acid (aa) seven-transmembrane receptor putative glycoprotein that binds the lysolipid phosphoric acid mediator, sphingosine 1-phosphate. Extracellular portions of human S1P2 show 82.5% aa identity with mouse S1P2 and 92% aa identity with human EDG-3, the most closely related family member. Both are expressed predominantly in the lung, heart, kidney, liver, spleen, thymus, testis and brain, mediating both mitogenic and anti-apoptotic effects.
  • Long Name:
    Endothelial Differentiation Gene 5
  • Entrez Gene IDs:
    9294 (Human); 14739 (Mouse); 29415 (Rat)
  • Alternate Names:
    AGR16; EDG5; EDG-5; endothelial differentiation G-protein coupled receptor 5; endothelial differentiation, sphingolipid G-protein-coupled receptor, 5; GPCR13; GPCR18; H218; LPB2; S1P receptor 2; S1P receptor EDG5; S1P receptor Edg-5; S1P2; S1PR2; sphingosine 1-phosphate receptor 2; sphingosine 1-phosphate receptor Edg-5; sphingosine-1-phosphate receptor 2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 8  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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