Human VSTM2A Alexa Fluor® 405-conjugated Antibody

Catalog #: FAB100371V Datasheet / COA / SDS
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Human VSTM2A Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Detects human VSTM2A in direct ELISAs.
Monoclonal Mouse IgG2B Clone # 1024735
Protein A or G purified from hybridoma culture supernatant
Human embryonic kidney cell HEK293-derived human VSTM2A protein
Accession # Q8TAG5-2
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)


Recommended Concentration
Flow Cytometry
0.25-1 µg/106 cells
HEK293 Human Cell Line transfected with Human VSTM2A and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: VSTM2A

V-set and transmembrane domain-containing protein 2A (VSTM2A) is a secreted glycoprotein that is expressed by committed preadipocytes. N-linked glycosylation is crucial for its secretion, but not for preadipocyte cell differentiation activity. It is expressed during adipocyte development and its over-expression promotes adipogenesis (1). VSTM2A is highly expressed in the brain and Vstm2a was identified as an enigmatic gene that is highly produced in mouse brain (1, 2). A positive association has been observed between Vstm2a and Pparg2. VSTM2A plays a role in the regulation of the early stage of white and brown preadipocyte cell differentiation. It promotes adipogenic commitment of preadipocytes by increasing gene expression of the transcription factor PPARG in a BMP4-dependent signaling pathway (1, 3). In humans, two isoforms (1 and 2) exist due to alternative splicing. Human VSTM2A is synthesized either as a 236 amino acid (aa) (Isoform 1) or a 244 aa (Isoform 2) precursor that contains a 24 aa signal sequence followed by the VSTM2A domain. Human Isoform 2 VSTM2A shares 78% aa sequence identity with mouse and rat VSTM2A.

  1. Secco, B. et al. (2017) Cell Rep. 18:93.
  2. Pandey, A.K. et al. (2014) PloS One. 9:e88889.
  3. Berry, D.C. et al. (2013) Development. 140:3939.
Long Name
V-Set And Transmembrane Domain Containing 2A
Entrez Gene IDs
222008 (Human); 211739 (Mouse); 689106 (Rat)
Alternate Names
MGC33530; V-set and transmembrane domain containing 2; V-set and transmembrane domain containing 2A; V-set and transmembrane domain-containing protein 2A; VSTM2; VSTM2A

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Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.


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