Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1024735
View all VSTM2A Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell HEK293-derived human VSTM2A protein
Ser25-Phe244
Accession # Q8TAG5-2

Specificity

Detects human VSTM2A in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human VSTM2A Antibody

Detection of VSTM2A antibody in HEK293 Human Cell Line transfected with Human VSTM2A and eGFP antibody by Flow Cytometry

Detection of VSTM2A in HEK293 Human Cell Line transfected with Human VSTM2A and eGFP by Flow Cytometry

HEK293 human embryonic kidney cell line transfected with either (A) human VSTM2A or (B) irrelevant protein, and eGFP, was stained with Mouse anti-human VSTM2A monoclonal antibody (Catalog # MAB100371) followed by Allophycocyanin-conjugated anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB10041, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Human VSTM2A ELISA Standard Curve.

Recombinant Human VSTM2A protein was serially diluted 2-fold and captured by Mouse Anti-Human VSTM2A Monoclonal Antibody (MAB10037) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human VSTM2A Monoclonal Antibody (Catalog # MAB100371) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Applications for Human VSTM2A Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human VSTM2A Monoclonal Antibody (Catalog # MAB10037).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line transfected with Human VSTM2A and eGFP

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VSTM2A

V-set and transmembrane domain-containing protein 2A (VSTM2A) is a secreted glycoprotein that is expressed by committed preadipocytes. N-linked glycosylation is crucial for its secretion, but not for preadipocyte cell differentiation activity. It is expressed during adipocyte development and its over-expression promotes adipogenesis (1). VSTM2A is highly expressed in the brain and Vstm2a was identified as an enigmatic gene that is highly produced in mouse brain (1, 2). A positive association has been observed between Vstm2a and Pparg2. VSTM2A plays a role in the regulation of the early stage of white and brown preadipocyte cell differentiation. It promotes adipogenic commitment of preadipocytes by increasing gene expression of the transcription factor PPARG in a BMP4-dependent signaling pathway (1, 3). In humans, two isoforms (1 and 2) exist due to alternative splicing. Human VSTM2A is synthesized either as a 236 amino acid (aa) (Isoform 1) or a 244 aa (Isoform 2) precursor that contains a 24 aa signal sequence followed by the VSTM2A domain. Human Isoform 2 VSTM2A shares 78% aa sequence identity with mouse and rat VSTM2A.

References

  1. Secco, B. et al. (2017) Cell Rep. 18:93.
  2. Pandey, A.K. et al. (2014) PloS One. 9:e88889.
  3. Berry, D.C. et al. (2013) Development. 140:3939.

Long Name

V-Set And Transmembrane Domain Containing 2A

Alternate Names

VSTM2

Entrez Gene IDs

222008 (Human); 211739 (Mouse); 689106 (Rat)

Gene Symbol

VSTM2A

UniProt

Q8TAG5-2

Additional VSTM2A Products

Product Documents for Human VSTM2A Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human VSTM2A Antibody

For research use only

Related Research Areas

Adipogenesis Markers

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Protocols

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