Human VSTM2A Antibody
Human VSTM2A Antibody Summary
Accession # Q8TAG5-2
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human VSTM2A Monoclonal Antibody (Catalog # MAB10037).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of VSTM2A in HEK293 Human Cell Line transfected with Human VSTM2A and eGFP by Flow Cytometry HEK293 human embryonic kidney cell line transfected with either (A) human VSTM2A or (B) irrelevant protein, and eGFP, was stained with Mouse anti-human VSTM2A monoclonal antibody (Catalog # MAB100371) followed by Allophycocyanin-conjugated anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB10041, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol.
Human VSTM2A ELISA Standard Curve. Recombinant Human VSTM2A protein was serially diluted 2-fold and captured by Mouse Anti-Human VSTM2A Monoclonal Antibody (MAB10037) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human VSTM2A Monoclonal Antibody (Catalog # MAB100371) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
V-set and transmembrane domain-containing protein 2A (VSTM2A) is a secreted glycoprotein that is expressed by committed preadipocytes. N-linked glycosylation is crucial for its secretion, but not for preadipocyte cell differentiation activity. It is expressed during adipocyte development and its over-expression promotes adipogenesis (1). VSTM2A is highly expressed in the brain and Vstm2a was identified as an enigmatic gene that is highly produced in mouse brain (1, 2). A positive association has been observed between Vstm2a and Pparg2. VSTM2A plays a role in the regulation of the early stage of white and brown preadipocyte cell differentiation. It promotes adipogenic commitment of preadipocytes by increasing gene expression of the transcription factor PPARG in a BMP4-dependent signaling pathway (1, 3). In humans, two isoforms (1 and 2) exist due to alternative splicing. Human VSTM2A is synthesized either as a 236 amino acid (aa) (Isoform 1) or a 244 aa (Isoform 2) precursor that contains a 24 aa signal sequence followed by the VSTM2A domain. Human Isoform 2 VSTM2A shares 78% aa sequence identity with mouse and rat VSTM2A.
- Secco, B. et al. (2017) Cell Rep. 18:93.
- Pandey, A.K. et al. (2014) PloS One. 9:e88889.
- Berry, D.C. et al. (2013) Development. 140:3939.
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