Recombinant Human IGFBP-3 Protein

Novus Biologicals | Catalog # NBP2-35003

Novus Biologicals
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Key Product Details

Source

E. coli

Accession Number

P17936

Applications

Bioactivity, SDS-PAGE
View all IGFBP-3 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 264 amino acids corresponding to IGFBP-3 Source: E. coli

Uniprot ID: P17936

Amino Acid Sequence: GASSAGLGPV VRCEPCDARA LAQCAPPPAV CAELVREPGC GCCLTCALSE GQPCGIYTER CGSGLRCQPS PDEARPLQAL LDGRGLCVNA SAVSRLRAYL LPAPPAPGNA SESEEDRSAG SVESPSVSST HRVSDPKFHP LHSKIIIIKK GHAKDSQRYK VDYESQSTDT QNFSSESKRE TEYGPCRREM EDTLNHLKFL NVLSPRGVHI PNCDKKGFYK KKQCRPSKGR KRGFCWCVDK YGQPLPGYTT KGKEDVHCYS MQSK

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IGFBP-3 as determined by LAL method.

Predicted Molecular Mass

28.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The ED50 as determined by inhibiting IGF-II induced proliferation of serum free human MCF-7 cells is less than 200 ng/ml, corresponding to a specific activity of > 5.0 x 10^3 IU/mg in the presence of 15 ng/ml of rHuIGF-II.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human IGFBP-3 Protein

SDS-PAGE: Recombinant Human IGFBP-3 Protein [NBP2-35003]

SDS-PAGE: Recombinant Human IGFBP-3 Protein [NBP2-35003]

SDS-Page: Human IGFBP-3 Protein [NBP2-35003]

Formulation, Preparation, and Storage

NBP2-35003
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IGFBP-3

Insulin-like Growth Factor-Binding Protein 3 (IGF-BP3) belongs to the IGFBP family, which are all cysteinerich proteins with conserved cysteine and have an IGFBP domain and a thyroglobulin type-I domain. Mature human IGF-BP3 contains 264 a.a. with three potential N-linked and two potential O-linked glycosylation sites. It is expressed by most tissues and has higher levels during extrauterine life and peak during puberty. The expression of IGF-BP3 in fibroblasts is stimulated by mitogenic growth factors such as Bombesin, Vasopressin, PDGF, and EGF. The protein forms a ternary complex with IGF-I or II and acid-labile subunit. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Decreased plasma levels of IGF-BP3 often happen during the progression of prostate cancer from benign to metastatic disease.

Long Name

Insulin-like Growth Factor Binding Protein 3

Alternate Names

IGFBP3

Gene Symbol

IGFBP3

UniProt

P17936

Additional IGFBP-3 Products

Product Documents for Recombinant Human IGFBP-3 Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human IGFBP-3 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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