ImmunoFect STAR™ Antibody Transfection Reagent

Tocris Bioscience | Catalog # 8911

Reagent capable of efficiently delivering functional antibodies into the cytosol of cells
Tocris Bioscience

Key Product Details

Description

Reagent capable of efficiently delivering functional antibodies into the cytosol of cells
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Product Description

ImmunoFect STAR™ Antibody Transfection Reagent is a highly efficient antibody transfection reagent designed for intracellular delivery of antibodies into live cells. ImmunoFect STAR™ Antibody Transfection Reagent enables functional studies of intracellular targets by facilitating direct antibody access to the cytosol. The delivery mechanism is optimized for high efficiency while maintaining cell viability.

Please see the protocol for further information on how to use ImmunoFect STAR™ Antibody Transfection Reagent.

This compound is supplied pre-dissolved in ethanol (10 mg/mL).

ImmunoFect STAR is a trademark of Bio-Techne Corp.

Scientific Data Images for ImmunoFect STAR™ Antibody Transfection Reagent

ImmunoFect STAR™ Antibody Transfection Reagent applications

Application of ImmunoFect STAR™ Antibody Transfection Reagent.

Cytosolic delivery of anti-NUP153 antibody targeting the nuclear pore complex. HeLa cells were treated with anti-NUP153/ ImmunoFect STAR™ reagent complexes for 24 h, anti-NUP153 10 μg/mL, and ImmunoFect STAR™ reagent was 8 μg/mL). Cells were then washed, permeabilized and stained with AF647 conjugated anti-rabbit IgG and Hoechst before imaging and colocalization measurements.
ImmunoFect STAR™ Antibody Transfection Reagent applications

Application of ImmunoFect STAR™ Antibody Transfection Reagent.

Cytosolic delivery of native antibodies, fluorescence imaging was conducted after HeLa cells were treated with IgG-FITC/ ImmunoFect STAR™ reagent complexes for 4 h, IgG-FITC was 8 μg/mL, and ImmunoFect STAR™ reagent was 8 μg/mL (n = 3). Scale bar, 25 μm.

Product Specifications for ImmunoFect STAR™ Antibody Transfection Reagent

Storage

Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solubility Soluble in ethanol (supplied pre-dissolved in anhydrous ethanol, 10mg/ml)

Calculators

Calculate the dilution required to prepare a stock solution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background References

References are publications that support the biological activity of the product.

Product Documents for ImmunoFect STAR™ Antibody Transfection Reagent

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for ImmunoFect STAR™ Antibody Transfection Reagent

For research use only

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