Integrin alpha E/CD103 Antibody (2E7) [PE]

Name: Integrin alpha E/CD103 Antibody (2E7) [PE]
Brand: Novus Biologicals
SKU: NBP1-28126
Price: 529 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-28126

Submit a review

Product Documents

View Available Conjugates View Available Formulations

100% Guarantee

Product Specifications
Scientific Data
Applications
Spectra Viewer
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Monoclonal Armenian Hamster IgG₂ Clone # 2E7

View all Integrin alpha E/CD103 Primary Antibodies »

Product Specifications

Immunogen

C57BL/6J mouse intestinal intraepithelial lymphocytes (IEL)

Specificity

The CD103/alpha IELbeta7 integrin complex (Mr 160 and 115 kDa peptides).

Clonality

Monoclonal

Host

Armenian Hamster

Isotype

IgG₂

Scientific Data Images for Integrin alpha E/CD103 Antibody (2E7) [PE]

Flow Cytometry: Integrin alpha E/CD103 Antibody (2E7) [PE] [NBP1-28126] - BALB/c mouse mesenteric lymph node cells were stained with Hamster Anti-Mouse CD103-PE and Rat Anti-Mouse CD3 epilson-FITC.

Applications for Integrin alpha E/CD103 Antibody (2E7) [PE]

Application

Recommended Usage

Flow Cytometry

0.2 ug/10^6 cells

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS and a stabilizing agent

Preservative

Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Integrin alpha E/CD103

CD103/Integrin alpha E is a member of the integrin series of adhesion molecules. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and approx. 20% of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs, appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis and periphery. (1-3) In non-epithelial CD103+ T cells there is a bias toward expression on CD8+.cells. The 2E7 mAb exhibits antigen immunoprecipitation patterns similar if not identical to the rat anti-mouse CD103 mAb M290. (1) The 2E7 mAb is reported to have signal-inducing activity in a redirected lysis assay and to co-stimulate IEL and CD8+ lymph node cells in conjunction with anti-TCR in vitro, which runs counter to the usual inhibition of CTL by anti-integrins. (1) This co-stimulatory activity is also shared by the mAb M290. (4) The property of costimulation is not unique to the mouse since the combination of anti-human alpha IEL plus anti-TCR will produce similar proliferation in human cells, with the exception that CD8+ cells are not stimulated. (5)

Alternate Names

CD103, HUMINAE, ITGAE

Gene Symbol

ITGAE

Additional Integrin alpha E/CD103 Products

Product Documents for Integrin alpha E/CD103 Antibody (2E7) [PE]

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Integrin alpha E/CD103 Antibody (2E7) [PE]

Protect conjugated forms from light.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.